18家企业阿胶中氨基酸的含量分析与比较研究
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篇名: 18家企业阿胶中氨基酸的含量分析与比较研究
TITLE:
摘要: 目的:为阿胶的质量评价提供参考。方法:采用全自动氨基酸分析仪分别测定18家企业共18批次阿胶中17种氨基酸的含量,再对含量数据进行主成分分析和聚类分析,以了解各种氨基酸之间的联系,并将相似的阿胶产品进行集合与分类。结果:除1批次阿胶外,其余17批次均含有17种目标氨基酸,包括苏氨酸(Thr)、缬氨酸(Val)、甲硫氨酸(Met)、异亮氨酸(Ile)、亮氨酸(Leu)、苯丙氨酸(Phe)和赖氨酸(Lys)7种人体必需氨基酸,尤其以甘氨酸(Gly)和脯氨酸(Pro)的含量较高;氨基酸总含量介于66.1%~82.0%之间,相差较大。主成分分析提取得到4个主成分,累积贡献率达到89.578%,并认为Pro、丙氨酸(Ala)、谷氨酸(Glu)、天冬氨酸(Asp)、Leu、Ile、Thr是市售阿胶的特征氨基酸。聚类分析得到表征3张市售阿胶树系图,欧式距离系数均在0~25之间变动。结论:市售阿胶中氨基酸总含量相差较大,且不同企业阿胶中各种氨基酸的含量也有一定差异。阿胶中蛋白质成分主要为胶原蛋白,可以利用测定氨基酸含量来监控阿胶质量。
ABSTRACT: OBJECTIVE: To provide reference for quality evaluation of Ejiao. METHODS: The contents of 17 amino acids in 18 batches of Ejiao from 18 manufacturers were analyzed by automatic amino acid analyzer; the content data was processed by principal component analysis (PCA) and hierarchical clustering analysis (HCA), in order to understand the relationship among these amino acids and categorize the Ejiao products. RESULTS: Except for a batch of Ejiao, the other 17 batches contained 17 various amino acids, including 7 necessary amino acids (Thr, Val, Met, Ile, Leu, Phe and Lys) for human being, especially the contents of Gly and Pro were higher. The total content of amino acids was arranged from 66.1% to 82.0%, showing great difference. Four main components were extracted by PCA, the cumulative contribution of which was 89.578%, and accordingly it may be considered that Pro, Ala, Glu, Asp, Leu, Ile and Thr were characteristic amino acids of Ejiao products. In hierarchical clustering analysis, 3 tree figures of commercial Ejiao product were achieved and Euclidean distance was varied among 0-25. CONCLUSIONS: There is great difference in total control of amino acid in commercial Ejiao product; Ejiao products from various manufacturers differ in their amino acid contents. Ejiao is rich in collagen, and therefore, using amino acid determination would be helpful to monitor its quality.
期刊: 2017年第28卷第1期
作者: 葛重宇,庞慧,李楠,郑洁,何昌隆,杨欢,沈玉萍
AUTHORS: GE Chongyu,PANG Hui,LI Nan,ZHENG Jie,HE Changlong,YANG Huan,SHEN Yuping
关键字: 阿胶;氨基酸;主成分分析;聚类分析
KEYWORDS: Ejiao; Amino acids; Principal component analysis; Hierarchical clustering analysis
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