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蒙药山野豌豆多糖的提取与含量测定
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| 篇名： | 蒙药山野豌豆多糖的提取与含量测定 |
| TITLE： | |
| 摘要： | 目的：优化蒙药山野豌豆多糖提取液经活性炭脱色的条件，并建立其提取及含量测定方法。方法：以水提醇沉法提取蒙药山野豌豆粗多糖。以脱色率为考察指标，设计正交试验，考察活性炭用量、脱色时间及脱色温度对粗多糖脱色的影响，优化活性炭脱色条件。以葡萄糖为对照品，采用苯酚-硫酸法，490 nm波长下经紫外分光光度法测定粗多糖中多糖含量。结果：优化的活性炭脱色条件为活性炭用量3％、脱色时间40 min、脱色温度60 ℃。以此条件对粗多糖进行脱色验证试验，平均脱色率可达19.77％（RSD＝1.85％，n＝3）。蒙药山野豌豆粗多糖平均提取得率为4.56％（RSD＝2.38％，n＝3），提取液中多糖含量为1.98％（RSD＝2.18％，n＝4）。结论：本试验提取多糖得率重复性较好；建立的多糖含量测定方法稳定、可行。 |
| ABSTRACT： | OBJECTIVE： To optimize the decolorization condition for polysaccharide extract of Mongolian medicine Vicia amoena， and to establish the method for its extraction and content determination. METHODS： The water extract-alcohol precipitation was used to extract polysaccharide from Mongolian medicine V. amoena. Using decolorization rate as index， orthogonal test was designed to investigate the effects of the dosage of activated carbon， decolorization temperature， decolorization time on the decolorization of polysaccharide， so as to optimize the conditions for the decoloration of polysaccharide. Using glucose as control， phenol sulfuric acid method was adopted， and the content of polysaccharide in crude polysaccharide was determined by UV spectrophotometry at 490 nm. RESULTS： The optimal decoloration condition was as follows as actived carbon of 3％， decoloration time of 40 min， decoloration temperature of 60 ℃. On this basis， the average decolorization rate reached 19.77％（RSD＝1.85％，n＝3） by the verification test of the decoloration. The average extraction yield for the crude polysaccharide was 4.56％（RSD＝2.38％，n＝3）， of which the polysaccharide content was 1.98％（RSD＝2.18％，n＝4）. CONCLUSIONS： This experiment has relatively good reproducibility in polysaccharide yield； established method for content determination of polysaccharide is stable and feasible. |
| 期刊： | 2017年第28卷第4期 |
| 作者： | 李振，云学英，于海青，李成林，郭晓宇，况媛媛 |
| AUTHORS： | LI Zhen，YUN Xueying，YU Haiqing，LI Chenglin，GUO Xiaoyu，KUANG Yuanyuan |
| 关键字： | 蒙药；山野豌豆；多糖；分光光度法；含量测定；活性炭；脱色率；正交试验 |
| KEYWORDS： | Mongolian medicine； Vicia amoena； Polysaccharide； Spectrophotometry； Content determination； Activated carbon； Decolorization rate； Orthogonal test |
| 阅读数： | 294 次 |
| 本月下载数： | 15 次 |
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