LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究
x
请在关注微信后,向客服人员索取文件
篇名: | LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究 |
TITLE: | |
摘要: | 目的:比较液相色谱-串联质谱(LC-MS/MS)法与酶放大免疫(EMIT)法测定人血清中丙戊酸(VPA)浓度的差异,为临床治疗药物监测提供参考。方法:分别采用LC-MS/MS法和EMIT法对144份住院或门诊患者血清样品中的VPA浓度进行检测,采用配对t检验、Pearson相关性分析和Bland-Altman偏差图等方法对其检测结果的差异进行评价。结果:LC-MS/MS法和EMIT法的检测结果呈正相关(r=0.924,P<0.05);两者的回归方程为cEMIT=0.920 7cLC-MS/MS-1.114 4(r=0.924);LC-MS/MS法和EMIT法测得的VPA平均血药浓度分别为(49.9±21.2)、(54.9±21.3)μg/mL,组间比较差异有统计学意义(P<0.05);EMIT法检测结果比LC-MS/MS法偏高8.3 μg/mL,95%置信区间为(-13.6,18.7)。结论:LC-MS/MS法和EMIT法测定人血清中VPA的浓度相关性良好,但检测结果有一定的差异,建议同一患者长期监测应选用同一种方法。 |
ABSTRACT: | OBJECTIVE: To compare the difference in the concentration determination of valproic acid (VPA) in human serum by LC-MS/MS and EMIT. METHODS: Both LC-MS/MS and EMIT methods were applied to determine the serum concentration of VPA in 144 inpatients or outpatients. The paired t-test, Pearson correlation analysis, Bland-Altman deviation chart and other methods were used to evaluate the difference in the results of concentration determination. RESULTS: The results of LC-MS/MS method was positively correlated with that of EMIT method (r=0.924,P<0.05); the regression equation of them was cEMIT=0.920 7cLC-MS/MS-1.114 4(r=0.924). Average serum concentrations of VPA determined by LC-MS/MS and EMIT were (49.9±21.2) and (54.9±21.3) μg/mL, with statistical significance (P<0.05). The serum concentration of VPA determined by EMIT was higher than that by LC-MS/MS 8.3 μg/mL, 95% confidence interval was (-13.6,18.7). CONCLUSIONS: The serum concentration of VPA determined by LC-MS/MS and EMIT have high correlation. But the determination results still have certain difference, it is suggested to use same method for long- term monitering. |
期刊: | 2017年第28卷第8期 |
作者: | 时海浪,王海东,宋兴发 |
AUTHORS: | SHI Hailang,WANG Haidong,SONG Xingfa |
关键字: | 丙戊酸;血药浓度;液相色谱-串联质谱法;酶放大免疫法;差异 |
KEYWORDS: | Valproic acid; Serum concentration; LC-MS/MS; EMIT; Difference |
阅读数: | 437 次 |
本月下载数: | 18 次 |
* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!