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丹参酮对人工关节无菌性松动小鼠气囊模型中炎症反应的影响
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| 篇名： | 丹参酮对人工关节无菌性松动小鼠气囊模型中炎症反应的影响 |
| TITLE： | |
| 摘要： | 目的：研究丹参酮对人工关节无菌性松动小鼠气囊模型中炎症反应的影响。方法：将小鼠随机分为空白对照组（生理盐水）、钛颗粒组（生理盐水）和丹参酮低、中、高剂量组（50、100、200 mg/kg），每组10只。所有小鼠建立气囊模型，除空白对照组小鼠气囊内注入0.5 mL生理盐水外，其余各组小鼠气囊内均注入0.5 mL钛颗粒悬液（10 mg/mL），24 h后按0.1 mL/10 g连续ig相应药物14 d。末次给药24 h后收集气囊，肉眼和苏木精-伊红染色显微镜观察气囊炎症情况，计算炎症细胞密度；实时定量聚合酶链式反应法检测气囊组织中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）mRNA表达量；酶联免疫吸附法检测气囊组织中TNF-α、IL-1β蛋白表达量。结果：与空白对照组比较，钛颗粒组小鼠气囊红肿明显，可见较多渗出及新生血管，炎症反应严重，炎症细胞密度明显增加（P＜0.05）；TNF-α、IL-1β mRNA及蛋白表达明显增强（P＜0.05）。与钛颗粒组比较，丹参酮各剂量组小鼠气囊红肿减轻，渗出及新生血管减少，炎症反应减轻，炎症细胞密度明显减小（P＜0.05）；TNF-α、IL-1β mRNA及蛋白表达明显减弱（P＜0.05），且呈剂量依赖性。结论：丹参酮可有效抑制人工关节无菌性松动小鼠气囊模型中的无菌性炎症反应。 |
| ABSTRACT： | OBJECTIVE： To study the effect of tanshinone on inflammatory response in air-pouch model mice with artificial joint aseptic loosening. METHODS： Mice were randomly divided into blank control group （normal saline）， titanium particle group （normal saline）， tanshinone low-dose， medium-dose， high-dose groups （50， 100， 200 mg/kg）， 10 in each group. Air-pouch models were induced. Except that mice were injected 0.5 mL normal saline into air-pouch in blank control group， other groups were injected 0.5 mL Titanium particle suspension （10 mg/mL） into air-pouch， continuously administrated medicines by 0.1 mL/10 g after 24 h， ig， for 14 d. After 24 h of last administration， the air-pouch was collected， air-pouch inflammation was observed by eyes and by microscopy after hematoxylin-eosin staining， and inflammatory cell density was calculated. Real-time quantitative polymerase chain reaction method was conducted to detect the tumor necrosis factor α （TNF-α）， interleukin 1β （IL-1β） mRNA expression； enzyme-linked immunosorbent method was used to detect the TNF-α， IL-1β protein expression. RESULTS： Compared with blank control group， air-pouch swelling was obvious in titanium particle group， much exudation and neovascular were observed， inflammatory response was severe， inflammatory cell density was increased significantly （P＜0.05）； TNF-α， IL-1β mRNA and protein expression were obviously enhanced （P＜0.05）. Compared with titanium particle group， air-pouch swelling was relieved in tanshinone doses groups， exudation and neovascular were decreased， inflammatory response was relieved， inflammatory cell density was decreased significantly （P＜0.05）； TNF-α， IL-1β mRNA and protein expression were obviously decreased （P＜0.05）， with a dose-dependent manner. CONCLUSIONS： Tanshinone can effectively inhibit the aseptic inflammatory response in air-pouch model mice with artificial joint aseptic loosening. |
| 期刊： | 2017年第28卷第13期 |
| 作者： | 赖笑雨，陈金财，刘训志，高辉，何澄 |
| AUTHORS： | LAI Xiaoyu，CHEN Jincai，LIU Xunzhi，GAO Hui，HE Cheng |
| 关键字： | 丹参酮；无菌性松动；气囊模型；炎性因子；小鼠 |
| KEYWORDS： | Tanshinone； Aseptic loosening； Air-pouch model； Inflammatory factor； Mice |
| 阅读数： | 383 次 |
| 本月下载数： | 3 次 |
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