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新疆一枝蒿有效部位主要成分的制备与鉴定
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| 篇名： | 新疆一枝蒿有效部位主要成分的制备与鉴定 |
| TITLE： | |
| 摘要： | 目的：建立新疆一枝蒿有效部位主要成分的快速识别和高效制备的方法，为民族药的研究提供参考。方法：采用液相-高分辨质谱（LC-HRMS/MS）法对一枝蒿有效部位的主要成分进行初步推测，利用高效液相色谱、紫外光谱和质谱法将部分化合物与相应对照品进行比对分析确定其名称；对尚未确定的化合物采用柱分离及制备液相色谱技术快速获得单体，并确定其结构。结果：从一枝蒿有效部位分离出5个化合物，鉴定出其中2个化合物为艾黄素和紫花牡荆素；获得1个单体化合物（得率为0.35 mg/g，纯度为98.5％），经结构确证为6-去甲氧基-4′ -O-甲基茵陈色原酮-7-O-β-D-葡萄糖苷。结论：本试验建立的方法实现了目标成分的快速分离、鉴定、制备，可应用于民族药复杂的物质基础研究。 |
| ABSTRACT： | OBJECTIVE： To establish a method for rapid identification and efficient preparation of main ingredients in effective parts of Xinjiang Artemisia rupestris， and provide reference for researching the ethnic medicines. METHODS： LC-HRMS/MS was conducted for the preliminary study of main ingredients in effective parts of A. rupestris. HPLC， UV and MS were used to compare and analyze parts of the compounds and its reference substances， their names were determined. Column separation and preparative liquid chromatography were used for the undetermined compounds to receive monomer rapidly， and the structures were identified. RESULTS： 5 compounds were separated from the effective parts， 2 of which were identified as artemetin and casticin. A monomeric compound was obtained （yield was 0.35 mg/g， the purity was 98.5％）， which was confirmed to be 6-demethoxy-4′ -O-methoxy-capillarisin-7-O-β-D-glucoside by the structure. CONCLUSIONS： The method has achieved rapid separation， identification and preparation of target ingredients， which can be used for the fundamental research of ethnic medicine complex materials. |
| 期刊： | 2017年第28卷第16期 |
| 作者： | 戎晓娟，顾政一，贺金华，杨璐 |
| AUTHORS： | RONG Xiaojuan，GU Zhengyi，HE Jinhua，YANG Lu |
| 关键字： | 一枝蒿；液质联用；制备液相色谱；分离；鉴定；单体制备；结构确证 |
| KEYWORDS： | Artemisia rupestris； LC-MS； Preparative liquid chromatography； Separation； Identification； Monomer preparation； Structure identification |
| 阅读数： | 386 次 |
| 本月下载数： | 4 次 |
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