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莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制研究
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| 篇名： | 莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制。方法：将大鼠随机分为正常组、模型组、阳性组（米非司酮，2.25 mg/kg）和药对合煎液组（莪术-三棱药对合煎液，6.0 g/kg），每组10只。除正常组外，其余各组大鼠分别于每周一、三、五im苯甲酸雌二醇注射液（0.5 mg/kg），持续12周；从第13周开始，造模组大鼠增加im黄体酮注射液（5 mg/kg），并每天ig给药1次，至实验第16周。给药结束后，测定大鼠子宫系数，苏木精-伊红染色观察大鼠子宫病理变化并测定平滑肌厚度，免疫组化染色法测定大鼠子宫组织中转化生长因子β3（TGF-β3）和基质金属蛋白酶11（MMP-11）蛋白表达。结果：与正常组比较，模型组大鼠子宫系数升高、子宫发生明显病理变化、子宫平滑肌厚度增加、子宫组织中TGF-β3和MMP-11蛋白表达增强（P＜0.05或 P＜0.01）；与模型组比较，阳性组和药对合煎液组大鼠上述变化均显著改善（P＜0.05或P＜0.01）。结论：莪术-三棱药对合煎液对大鼠子宫肌瘤有一定的防治作用，其机制可能与下调子宫组织中TGF-β3、MMP-11蛋白表达有关。 |
| ABSTRACT： | OBJECTIVE： To study the preventive and therapeutic effect and its mechanism of medicine pair decoction liquid of Curcuma phaeocaulis-Sparganium stoloniferum on rats with uterine myoma. METHODS： Rats were randomly divided into normal group， model group， positive group （mifepristone，2.25 mg/kg） and medicine pair decoction liquid group （C. phaeocaulis-S. stoloniferum decoction liquid， 6.0 g/kg）， 10 in each group. Except for the normal group， rats in other groups were injected Estradiol benzoate injection （0.5 mg/kg） intramuscularly every Monday， Wednesday and Friday， for 12 weeks. From the 13th week， rats in modeling group were added Progesterone injection （5 mg/kg） intramuscularly as well as relevant medicines intragastrically， once a day， until the 16th week. After administration， uterine coefficient of rats was detected. HE staining was used to observe the pathological changes of uterus and determine the thickness of smooth muscle； immunohistochemical staining was adopted to detect the transforming growth factor β3 （TGF-β3）， matrix metalloproteinase 11 （MMP-11） protein expressions in uterus tissue of rats. RESULTS： Compared with normal group， uterine coefficient was increased in model group， pathological changes were obvious in uterus， thickness of smooth muscle was increased， TGF-β3 and MMP-11 protein expressions were enhanced （P＜0.05 or P＜0.01）. Compared with model group， above-mentioned changes were improved significantly in positive group and medicine pair decoction liquid groups （P＜0.05 or P＜0.01）. CONCLUSIONS： Medicine pair decoction liquid of C. phaeocaulis-S. stoloniferum shows certain preventive and therapeutic effect on rats with uterine myoma. The mechanism may be associated with downregulating the TGF-β3， MMP-11 protein expressions in uterus tissue. |
| 期刊： | 2017年第28卷第19期 |
| 作者： | 李文静，李雪岩，崔涛，蔡德富，樊丽，张昊，刘雷，洪博 |
| AUTHORS： | LI Wenjing，LI Xueyan，CUI Tao，CAI Defu，FAN Li，ZHANG Hao，LIU Lei，HONG Bo |
| 关键字： | 莪术；三棱；药对；子宫肌瘤；转化生长因子β3；基质金属蛋白酶11；大鼠 |
| KEYWORDS： | Curcuma phaeocaulis； Sparganium stoloniferum； Medicine pair； Uterine myoma； Transforming growth factor β3； Matrix metalloproteinase 11； Rats |
| 阅读数： | 447 次 |
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