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桉柠蒎肠溶软胶囊对脂多糖诱导小鼠急性肺损伤的改善作用
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| 篇名： | 桉柠蒎肠溶软胶囊对脂多糖诱导小鼠急性肺损伤的改善作用 |
| TITLE： | |
| 摘要： | 目的：研究桉柠蒎肠溶软胶囊对脂多糖（LPS）诱导小鼠急性肺损伤（ALI）的改善作用。方法：将60只小鼠随机分为空白对照组、模型对照组和桉柠蒎肠溶软胶囊低、中、高剂量组（100、300、900 mg/kg），每组12只。给药组小鼠ig相应药物，空白对照组和模型对照组小鼠ig等体积生理盐水（0.1 mL/10 g）。给药2 h后，除空白对照组外，其余各组小鼠均采用LPS雾化吸入的方法诱导ALI模型。造模6 h后，处死小鼠取肺泡灌洗液（BALF）和肺组织，镜下观察肺组织形态学变化；血细胞计数板计算BALF中总细胞数和瑞氏-吉姆萨染色后计算BALF中中性粒细胞数；二喹啉甲酸法检测BALF上清液中总蛋白浓度；酶联免疫吸附法检测BALF上清液中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）含量。结果：与空白对照组比较，模型对照组小鼠肺组织发生明显病理学损伤，肺水肿严重；BALF中总细胞数、中性粒细胞数和BALF上清液中总蛋白浓度以及TNF-α、IL-6含量均显著增加（P＜0.01）。与模型对照组比较，桉柠蒎肠溶软胶囊高剂量组小鼠肺组织病理损伤明显改善，BALF中总细胞数、中性粒细胞数和BALF上清液中总蛋白浓度以及TNF-α、IL-6含量均显著减少（P＜0.05）；其余各组差异无统计学意义（P＞0.05）。结论：高剂量桉柠蒎肠溶软胶囊能明显改善LPS诱导的小鼠ALI。 |
| ABSTRACT： | OBJECTIVE： To investigate the improvement effect of Eucalyptol enteric soft capsule on mice with lipopolysaccharide（LPS）-induced acute lung injury （ALI）. METHODS： 60 mice were randomly divided into blank control group， model control group， Eucalyptol enteric soft capsule low-dose， medium-dose， high-dose groups （100， 300， 900 mg/kg）， 12 in each group. Mice in administration groups were intragastrically given relevant medicines， mice in blank control group and model control group were intragastrically given equal volume of normal saline （0.1 mL/10 g）. After 2 h of administration， except for the blank control group， ALI was induced in other groups by atomized LPS. After 6 h of modeling， the mice were sacrificed， alveolar lavage fluid （BALF） and lung tissue were taken. Morphological changes of lung tissue were observed under microscope； number of total cells， neutrophils in BALF were calculated by blood cell count plate and staining by wright-giemsa respectively. Total protein concentration in BALF supernatant was detected by BCA method； TNF-α， IL-6 contents in BALF supernatant were determined by enzyme-linked immunosorbent assay. RESULTS： Compared with blank control group， lung tissue of mice in model control group showed obvious pathological damage and severe pulmonary edema； number of total cells， neutrophils in BALF， total protein concentration and TNF-α， IL-6 contents in BALF supernatant were significantly increased （P＜0.01）. Compared with model control group， pathological damage in lung tissue of mice was obviously improved in Eucalyptol enteric soft capsule high-dose group， number of total cells， neutrophils in BALF， total protein concentration and TNF-α， IL-6 contents in BALF supernatant were significantly decreased （P＜0.05）； and there were no significant differences in other groups （P＞0.05）. CONCLUSIONS： High-dose Eucalyptol enteric soft capsule can obviously improve LPS-induced ALI of mice. |
| 期刊： | 2017年第28卷第19期 |
| 作者： | 李璇，杨玉梅 |
| AUTHORS： | LI Xuan，YANG Yumei |
| 关键字： | 桉柠蒎肠溶软胶囊；急性肺损伤；脂多糖；小鼠；改善作用 |
| KEYWORDS： | Eucalyptol enteric soft capsule； Acute lung injury； Lipopolysaccharide； Mice； Improvement effect |
| 阅读数： | 445 次 |
| 本月下载数： | 3 次 |
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