HPLC法同时测定黄芪药材中10种黄酮类成分的含量
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篇名: HPLC法同时测定黄芪药材中10种黄酮类成分的含量
TITLE:
摘要: 目的:建立同时测定黄芪药材中10种黄酮类成分含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent SB-C18,流动相为乙腈-0.3%甲酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为254 nm,柱温为35 ℃,进样量为10 μL。结果:毛蕊异黄酮-7-O-葡萄糖苷、异槲皮苷、染料木苷、刺芒柄花苷、毛蕊异黄酮、槲皮素、染料木素、山柰酚、异鼠李素和芒炳花素检测进样量线性范围分别为0.030 29~1.514 5 μg(r=0.999 4)、0.015 00~0.7 500  μg(r=0.999 5)、0.007 39~0.369 5 μg(r=0.999 1)、0.120 11~6.005 5 μg(r=0.999 8)、0.038 36~1.918 μg(r=0.999 9)、0.029 89~1.494 5 μg(r=0.999 5)、0.007 04~0.352 μg(r=0.999 4)、0.016 83~0.841 5 μg(r=0.999 5)、0.004 54~0.227 μg(r=0.999 9)、0.013 36~0.668 μg(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为99.55%~100.45%(RSD=0.36%,n=6)、99.34%~101.00%(RSD=0.59%,n=6)、98.05%~100.36%(RSD=1.27%,n=6)、99.73%~100.13%(RSD=0.18%,n=6)、99.70%~100.30%(RSD=0.22%,n=6)、99.67%~103.27%(RSD=1.37%,n=6)、98.13%~104.41%(RSD=2.37%,n=6)、96.35%~100.06%(RSD=1.46%,n=6)、99.47%~101.13%(RSD=0.60%,n=6)、99.70%~100.06%(RSD=0.15%,n=6)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于黄芪药材中10种黄酮类成分含量的同时测定。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of 10 flavonoids in Astragalus membranaceus. METHODS: HPLC method was adopted. The determination was performed on Agilent SB-C18 column with mobile phase consisted of acetonitrile-0.3% formic acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 254 nm, and the column temperature was 35 ℃. The sample size was 10 μL. RESULTS: The linear ranges of calycosin-7-O-glucoside, isoquercitrin, genistin, ononin, calycosin, quercetin, genistein, kaempferol, isorhamnetin and formononetion were 0.030 29-1.514 5 μg(r=0.999 4),0.015 00-0.7 500 μg(r=0.999 5),0.007 39-0.369 5 μg(r=0.999 1),0.120 11-6.005 5 μg(r=0.999 8),0.038 36-1.918 μg(r=0.999 9),0.029 89-1.494 5 μg(r=0.999 5),0.007 04-0.352 μg(r=0.999 4),0.016 83-0.841 5 μg(r=0.999 5),0.004 54-0.227 μg(r=0.999 9), 0.013 36-0.668 μg(r=0.999 9), respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 99.55%-100.45%(RSD=0.36%,n=6),99.34%-101.00%(RSD=0.59%,n=6), 98.05%-100.36%(RSD=1.27%,n=6), 99.73%-100.13%(RSD=0.18%,n=6),99.70%-100.30%(RSD=0.22%,n=6),99.67%-103.27%(RSD=1.37%,n=6), 98.13%-104.41%(RSD=2.37%,n=6),96.35%-100.06%(RSD=1.46%,n=6), 99.47%-101.13%(RSD=0.60%,n=6), 99.70%-100.06%(RSD=0.15%,n=6), respectively. CONCLUSIONS: This method is convenient, sensitive, stable and reproducible, can be used for simultaneous determination of 10 flavonoids in A. membranaceus.
期刊: 2017年第28卷第21期
作者: 张妍,董琳,雍婧姣,毛福英,尹蕾,付雪艳
AUTHORS: ZHANG Yan,DONG Lin,YONG Jingjiao,MAO Fuying,YIN Lei,FU Xueyan
关键字: 黄芪;黄酮类成分;含量测定;高效液相色谱法
KEYWORDS: Astragalus membranaceus; Flavonoids; Content determination;HPLC
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