壮药竹节蓼的质量标准研究
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篇名: 壮药竹节蓼的质量标准研究
TITLE:
摘要: 目的:建立壮药竹节蓼的质量标准。方法:采用薄层色谱法(TLC)对药材样品进行定性鉴别;采用高效液相色谱法对药材样品中槲皮苷进行含量测定:色谱柱为Agilent Zorbax SB-C18,流动相为乙腈-0.1%冰醋酸溶液(20 ∶ 80,V/V),流速为1.0 mL/min,检测波长为360 nm,柱温为30 ℃,进样量为10 μL;参照2015年版《中国药典》测定药材样品水分、灰分和浸出物的含量。结果:竹节蓼药材的TLC图斑点清晰,分离度好。槲皮苷检测质量浓度线性范围为0.023~0.46 mg/mL(r=0.999 1);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为98.01%~103.05%(RSD=1.71%,n=6)。药材样品水分、总灰分、酸不溶性灰分、浸出物和槲皮苷含量分别为6.39%~9.78%、2.82%~8.07%、0.02%~0.27%、14.14%~28.45%、0.09%~0.50%。结论:该研究所建标准可用于壮药竹节蓼的质量控制。
ABSTRACT: OBJECTIVE: To establish quality standard for Zhuang medicine Homalocladium platycladum. METHODS: TLC method was used for qualitative identification. HPLC method was used to determine the content of quercetrin in samples: Agilent Zorbax SB-C18 column, mobile phase consisted of acetonitrile-0.1% acetic acid (20 ∶ 80,V/V), flow rate of 1.0 mL/min, detection wavelength of 360 nm, column temperature of 30 ℃,injection volume of 10 μL. The contents of moisture, total ash and extract in samples were determined. referring to Chinese Pharmacopiea(2015 edition). RESULTS: TLC spots of H. platycladum were clear and well-separated without interference from negative control. The linear range of quercetin were 0.023-0.46 mg/mL(r=0.999 1). RSDs of precision, stability and repeatability tests were all lower than 2.0%. The average recoveries were 98.01%-103.05% (RSD=1.71%,n=6). The contents of moisture, total ash,extract and quercetrin in samples were 6.39%-9.78%, 2.82%-8.07%, 0.02%-0.27%, 14.14%-28.45%, 0.09%-0.50% respectively.  CONCLUSIONS: Established trial can be used for quality control of Zhuang medicine H. platycladum.
期刊: 2017年第28卷第21期
作者: 曾祥燕,唐玉荣,颜萍花,黎理
AUTHORS: ZENG Xiangyan,TANG Yurong,YAN Pinghua,LI Li
关键字: 壮药;竹节蓼;槲皮苷;定性;定量;薄层色谱法;高效液相色谱法
KEYWORDS: Zhuang medicine; Homalocladium platycladum; Quercetrin; Qualitative; Quantitative; TLC; HPLC
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