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黔产喜树叶的HPLC指纹图谱研究及聚类分析
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| 篇名： | 黔产喜树叶的HPLC指纹图谱研究及聚类分析 |
| TITLE： | |
| 摘要： | 目的：建立黔产喜树叶的高效液相色谱（HPLC）指纹图谱。方法：采用HPLC法。色谱柱为Gemini-NX C18，流动相为乙腈-0.2％磷酸溶液（梯度洗脱），流速为1.0 mL/min，检测波长为370 nm，柱温为30 ℃，进样量为10 μL。以山柰素为参照物，测定14批喜树叶药材的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》（2004 A）进行共有峰指认、相似度评价，并进行聚类分析。结果：14批喜树叶药材的HPLC 图谱共有10个共有峰， 13批喜树叶药材与对照图谱的相似度＞0.90，1批与对照图谱相似度＜0.90。喜树叶药材样品可归为3大类。结论：该研究所建指纹图谱可为黔产喜树叶的鉴别和质量评价提供参考。 |
| ABSTRACT： | OBJECTIVE： To establish HPLC fingerprint for the leaves of Camptotheca acuminante in Guizhou. METHODS： HPLC method was performed. The determination was performed on Gemini-NX C18 column with mobile phase consisted of acetonitrile-0.2％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 370 nm， and the column temperature maintained at 30 ℃. The sample size was 10 μL. Using sorbitol as a reference， HPLC fingerprints of 14 batches of the leaves of C. acuminante were determined. The chromatographic fingerprint was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A） in terms of common peak indentification， similarity evaluation and cluster analysis. RESULTS： There were 10 common peaks in HPLC fingerprints for 14 batches of the leaves of C. acuminate. And the similarity of 13 batches of the leaves of C. acuminate was greater than 0.90， and that of another one was less than 0.90. The leaves of C. acuminate were classified into 3 groups. CONCLUSIONS： The established fingerprint can provide reference for identification and quality evaluation of the leaves of C. acuminate. |
| 期刊： | 2017年第28卷第24期 |
| 作者： | 刘志海，范红梅，邹兰，隆林，金昭，余兰 |
| AUTHORS： | LIU Zhihai，FAN Hongmei，ZOU Lan，LONG Lin，JIN Zhao，YU Lan |
| 关键字： | 喜树叶；指纹图谱；聚类分析；高效液相色谱法 |
| KEYWORDS： | The leaves of Camptotheca acuminante； Fingerprint； Cluster analysis； HPLC |
| 阅读数： | 306 次 |
| 本月下载数： | 3 次 |
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