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白芍药材主根与根茎的HPLC指纹图谱比较研究
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| 篇名： | 白芍药材主根与根茎的HPLC指纹图谱比较研究 |
| TITLE： | |
| 摘要： | 目的：建立白芍药材主根与根茎的高效液相色谱（HPLC）指纹图谱，并比较两者的异同。方法：色谱柱为Phenomenex C18，流动相为乙腈-0.1％磷酸溶液（梯度洗脱），流速为1.0 mL/min，检测波长为230 nm，柱温为30 ℃，进样量为10 μL。以芍药苷为参照，测定白芍药材主根与根茎部分的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》（2012 版）进行共有峰指认和相似度评价。结果：白芍药材主根与根茎的HPLC图谱有9个共有峰；白芍药材主根与根茎之间相似度＞0.9。结论：该研究所建指纹图谱可为白芍药材的鉴别和质量评价提供参考，白芍药材主根与根茎有效成分一致，差异性较小。 |
| ABSTRACT： | OBJECTIVE： To establish HPLC fingerprints of taproot and rhizome of Paenoia lactiflora， and to compare the similarity and difference of them. METHODS： The determination was performed on Phenomenex C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 230 nm， and column temperature was 30 ℃. The sample size was 10 μL. Using paeoniflorin as reference， HPLC chromatograms of the taproot and rhizome of P. lactiflora were established. Common peak identification and similarity evaluation were performed by using TCM Chromatogram Fingerprint Similarity Evaluation System （2012 edition）. RESULTS： There were 9 common peaks in HPLC chromatograms of taproot and rhizome of P. lactiflora. The similarity of taproot with rhizome of P. lactiflora was higher than 0.9. CONCLUSIONS： Established fingerprints can provide reference for identification and quality evaluation of P. lactiflora. The effective constituent of taproot and rhizome of P. lactiflora are uniform but have small difference. |
| 期刊： | 2017年第28卷第27期 |
| 作者： | 宗志勇，郑金凤，路广义，秘砚鸿，苏爽，王景红 |
| AUTHORS： | ZONG Zhiyong，ZHENG Jinfeng，LU Guangyi，MI Xianhong，SU Shuang，WANG Jinghong |
| 关键字： | 白芍；主根；根茎；指纹图谱；相似度分析 |
| KEYWORDS： | Paenoia lactiflora； Taproot； Rhizome； Fingerprint； Similarity analysis |
| 阅读数： | 415 次 |
| 本月下载数： | 4 次 |
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