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阴炎净洗剂对大鼠接触性皮炎湿疹的防治作用及机制研究
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| 篇名： | 阴炎净洗剂对大鼠接触性皮炎湿疹的防治作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究阴炎净洗剂对大鼠接触性皮炎湿疹（ACD）的防治作用及机制。方法：将60只大鼠随机分为空白对照组、模型对照组、阳性对照组（地塞米松）和阴炎净洗剂高、中、低剂量组（分别给予2倍浓缩液、1倍浓缩液和原液），每组10只。除空白对照组外，其余各组大鼠均于腹、背（2 cm×2 cm）部涂布二硝基氯苯复制ACD模型。从造模第9天起，各给药组大鼠背部涂布相应药物100 μL，空白对照组和模型对照组大鼠涂布纯化水100 μL，每天给药3次，连续10 d。给药结束后，对皮肤变应性反应进行评分并测定皮肤肿胀度；采用苏木精-伊红染色观察皮肤病理变化；采用酶联免疫吸附法测定血清中白细胞介素2（IL-2）、IL-4、IL-6和干扰素γ（IFN-γ）含量。结果：与空白对照组比较，模型对照组大鼠变应性反应评分和皮肤肿胀度均显著升高（P＜0.01）；电镜下可见表皮结痂坏死、真皮层损伤严重、毛囊坏死；血清中IL-2、IL-4、IFN-γ含量显著增加（P＜0.05或P＜0.01），IL-6含量略有增加（P＞0.05）。与模型对照组比较，除阴炎净洗剂低剂量组大鼠皮肤肿胀度、血清中IL-4含量以及各给药组大鼠血清中IL-6含量改善不显著外，其余指标均显著改善（P＜0.05或P＜0.01）。结论：阴炎净洗剂对大鼠ACD具有一定的防治作用，其机制可能与降低血清中IL-2、IL-4、IFN-γ含量有关。 |
| ABSTRACT： | OBJECTIVE： To study the prevention and treatment effect and its mechanism of Yinyanjing lotion on allergic contact dermatitis （ACD） in rats. METHODS： 60 rats were randomly divided into blank control group， model control group， positive control group （dexamethasone） and Yinyanjing lotion high-dose， medium-dose， low-dose groups （respectively given 2-fold concentrated solution， 1-fold concentrated solution and original solution）， 10 in each group. Except for blank control group， rats in other groups smeared dinitrochlorobenzene in the abdomen and back （2 cm×2 cm） to induce ACD model. From the 9th of modeling， rats in each administration group were smeared 100 μL of related drugs in the back， and rats in blank control group and model control group smeared 100 μL of purified water， 3 times a day， for 10 d. After administration， skin allergic reaction was scored and skin swelling degree was determined. HE staining was used to observe the pathological changes of skin. Enzyme-linked immunosorbent method was used to detect the contents of interleukin-2 （IL-2）， IL-4， IL-6 and interferon γ （IFN-γ） in serum. RESULTS： Compared with blank control group， allergic reaction scores and skin swelling degree in model control group were significantly increased （P＜0.01）. Electron microscopy showed epidermal scab necrosis， dermal layer damage， hair follicle necrosis. Contents of IL-2， IL-4， IFN-γ in serum were significantly increased （P＜0.05 or P＜0.01）； IL-6 content was slightly increased （P＞0.05）. Compared with model control group， except that the skin swelling degree， IL-4 content in serum in Yinyanjing lotion low-dose group and IL-6 content in serum in each administration group were not significantly improved， other indexes were significantly improved （P＜0.05 or P＜0.01）. CONCLUSIONS： Yinyanjing lotion has certain prevention and treatment effects on ACD in rats， the mechanism may be associated with reducing the contents of IL-2， IL-4， IFN-γ in serum. |
| 期刊： | 2017年第28卷第28期 |
| 作者： | 肖作奇，欧阳波，肖素希，潘涛 |
| AUTHORS： | XIAO Zuoqi，OUYANG Bo，XIAO Suxi，PAN Tao |
| 关键字： | 阴炎净；接触性皮炎湿疹；白细胞介素；干扰素γ；大鼠 |
| KEYWORDS： | Yinyanjing； Allergic contact dermatitis； Interleukin； Interferon γ； Rats |
| 阅读数： | 363 次 |
| 本月下载数： | 16 次 |
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