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松贝和青贝的镇咳、平喘、化痰和抗炎作用比较研究
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| 篇名： | 松贝和青贝的镇咳、平喘、化痰和抗炎作用比较研究 |
| TITLE： | |
| 摘要： | 目的：考察松贝和青贝的镇咳、平喘、化痰和抗炎作用效果差异。方法：将小鼠、豚鼠随机分为空白对照组（蒸馏水），松贝和青贝的醇提物组、水提物组、粉末组（给药剂量小鼠为1.13 g/kg、豚鼠为0.42 g/kg，均以生药量计）以及阳性对照组，每组10只。各组动物均每天灌胃给药1次，连续给药5 d，末次给药30 min后进行相关指标检测。采用氨水引咳法，通过测定小鼠的咳嗽潜伏期和3 min内咳嗽次数来考察止咳作用（阳性药为氢溴酸右美沙芬，0.013 g/kg）；以2％磷酸组胺引喘15 s后，通过测定豚鼠的引喘潜伏期和抽搐跌倒只数来考察平喘作用（阳性药为氨茶碱，0.033 g/kg）；采用酚红注射法，通过测定小鼠气管中酚红含量来考察化痰作用（阳性药为盐酸氨溴索，21.304 g/kg）；采用二甲苯致炎实验，通过测定小鼠耳肿胀度和耳肿胀抑制率来考察抗炎作用（阳性药为醋酸地塞米松，9.225×10－4 g/kg）。结果：与空白对照组比较，松贝和青贝的醇提物组、粉末组以及阳性对照组小鼠的咳嗽潜伏期和引喘潜伏期显著延长、3 min内咳嗽次数显著减少，松贝粉末组、青贝醇提物组、青贝粉末组和阳性对照组抽搐跌倒豚鼠只数显著减少，各给药组小鼠气管中酚红含量显著增加、耳肿胀度和耳肿胀抑制率显著降低，差异均有统计学意义（P＜0.05或P＜0.01），且粉末组小鼠各指标与阳性对照组比较差异无统计学意义（P＞0.05）；与松贝相同组间比较，青贝醇提物组小鼠的咳嗽潜伏期显著延长（P＜0.05），其余指标差异无统计学意义（P＞0.05）。结论：松贝的镇咳、平喘、化痰和抗炎效果与青贝相当或稍弱。 |
| ABSTRACT： | OBJECTIVE： To investigate antitussive， antiasthmatic， phlegm-resolving and anti-inflammatory effects of Fritillaria cirrhosa and Fritillaria unibracteata. METHODS： Mice and guinea pig were randomly divided into blank control group （distilled water）， ethanol extract groups， water extraction groups and powder groups of F. cirrhosa and F. unibracteata （1.13 g/kg for mice， 0.42 g/kg for guinea pig， calculated by crude drug）， positive control group， with 10 mice （guinea pig） in each group. Each group was given relevant medicine intragastrically once a day for consecutive 5 d. Relative indicators were detected 30 min after last medication. The ammonia water induced cough method was used to investigate antitussive effect （dextromethorphan hydrobromide as positive drug， 0.013 g/kg） by determining coughing latent period and coughing times within 3 min. After inducing asthma with 2％ histamine phosphate for 15 s， antiasthmatic effect （aminophylline as positive drug， 0.033 g/kg） was investigated by determining coughing latent period and the number of guinea pig with convulsive fall. The phenol red injection method was used to investigate phlegm-resolving effect （ambroxol as positive drug， 21.304 g/kg） by determining the content of phenol red in the trachea of mice in phenol red expectoration test. The anti-inflammatory effect （dexamethasone acetate as positive drug， 9.225×10－4 g/kg） was investigated by determining ear swelling degree and inhibition rate of ear swelling mice in xylene-induced inflammation test. RESULTS： Compared with blank control group， coughing latent period and cough induction latent period prolonged significantly in ethanol extract of F. cirrhosa and F. unibracteata groups， F. cirrhosa and F. unibracteata powder groups， positive control group， and coughing time decreased significantly within 3 min. The number of mice with convulsive fall decreased significantly in F. cirrhosa powder group， F. unibracteata ethanol group， F. unibracteata powder group and positive control group. The content of phenol red in the trachea of mice increased significantly， while ear swelling and inhibitory rate of ear swelling decreased significantly， with statistical significance （P＜0.05 or P＜0.01）. There was no statistical significance in each index of mice between powder group and positive control group （P＞0.05）. Compared with corresponding groups of F. cirrhosa， coughing latent period of mice prolonged significantly in F. unibracteata ethanol extract group （P＜0.05）， other indexes had no statistical significance（P＞0.05）. CONCLUSIONS： The antitussive， antiasthmatic， phlegm-resolving and anti-inflammatory effects of F. cirrhosa are similar to or worse than those of F. unibracteata. |
| 期刊： | 2018年第29卷第11期 |
| 作者： | 李亚玲，张继，陈洪燕，李玲，张世波 |
| AUTHORS： | LI Yaling，ZHANG Ji，CHEN Hongyan，LI Ling，ZHANG Shibo |
| 关键字： | 松贝；青贝；镇咳作用；平喘作用；祛痰作用；抗炎作用；小鼠；豚鼠 |
| KEYWORDS： | Fritillaria cirrhosa；Fritillaria unibracteata；Antitussive effect； Antiasthmatic effect； Phlegm-resolving effect； Anti-inflammatory effect； Mice； Guinea pig |
| 阅读数： | 1033 次 |
| 本月下载数： | 2 次 |
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