HPLC法同时测定蒙药达乌里芯芭中梓醇和益母草苷的含量
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篇名: | HPLC法同时测定蒙药达乌里芯芭中梓醇和益母草苷的含量 |
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摘要: | 目的:建立同时测定蒙药达乌里芯芭中梓醇和益母草苷含量的方法。方法:采用高效液相色谱法。色谱柱为XSelect HSS T3 C18,流动相为乙腈-1.0%磷酸水溶液(梯度洗脱),流速为1 mL/min,柱温为40 ℃,检测波长为203 nm,进样量为10 μL。采用建立的方法测定7批达乌里芯芭样品中梓醇和益母草苷的含量。结果:梓醇、益母草苷进样量检测线性范围分别为0.17~3.4、0.25~5.0 μg(r均≥0.999 5),检测限分别为3.57、4.72 ng,定量限分别为12.24、41.98 ng,精密度、稳定性(24 h)、重复性试验的RSD均<2.0%(n=6),加样回收率分别为93.0%~97.3%、92.1%~97.9%(RSD分别为1.9%、2.4%,n=6)。7批样品中梓醇、益母草苷的含量分别为0.53~0.81、0.79~1.25 mg/g。结论:建立的方法快速简便、稳定可靠,可用于达乌里芯芭中梓醇和益母草苷含量的同时测定。 |
ABSTRACT: | OBJECTIVE: To develop a method for simultaneous determination of catalpol and leonuride in Mongolian medicine Cymbaria dahurica. METHODS: HPLC method was adopted. The determination was performed on XSelect HSS T3 C18 column with mobile phase consisted of acetonitrile-1.0% phosphoric acid water solution (gradient elution) at the flow rate of 1 mL/min. The column temperature was set at 40 ℃, and the detection wavelength was set at 203 nm. The sample size was 10 μL. Established method was adopted to determine the contents of catalpol and leonuride in 7 batches of C. dahurica samples. RESULTS: The linear range of catalpol and leonuride were 0.17-3.4 and 0.25-5.0 μg (r≥0.999 5). The detection limits were 3.57 and 4.72 ng, respectively. The quantitative limits were 12.24 and 41.98 ng, respectively. RSDs of precision, stability (24 h) and reproducibility tests were all lower than 2.0% (n=6). The average recoveries were 93.0%-97.3% (RSD=1.9%, n=6) and 92.1%-97.9% (RSD=2.4%, n=6). The contents of catalpol and leonuride in 7 batches of samples were 0.53-0.81 and 0.79-1.25 mg/g, respectively. CONCLUSIONS: The established method is rapid, simple, stable and reliable, and can be used for simultaneous determination of catalpol and leonuride in C. dahurica. |
期刊: | 2018年第29卷第11期 |
作者: | 包玉秋,那仁朝克图 |
AUTHORS: | BAO Yuqiu,Narenchaoketu |
关键字: | 蒙药;达乌里芯芭;高效液相色谱法;梓醇;益母草苷;含量测定 |
KEYWORDS: | Mongolian medicine; Cymbaria dahurica; HPLC; Catalpol; Leonuride; Content determination |
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