HPLC法同时测定鼻炎灵片中绿原酸、黄芩苷和欧前胡素的含量
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篇名: | HPLC法同时测定鼻炎灵片中绿原酸、黄芩苷和欧前胡素的含量 |
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摘要: | 目的:建立同时测定鼻炎灵片中绿原酸、黄芩苷和欧前胡素含量的方法。方法:采用高效液相色谱法。色谱柱为Waters SunFire C18,流动相为甲醇-0.20%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为280 nm,柱温为30 ℃,进样量为20 μL。结果:绿原酸、黄芩苷和欧前胡素的检测质量浓度线性范围分别为1.618~16.18、1.624~16.24、1.762~17.62 μg/mL(r均为0.999 9);定量限分别为2.67、2.03、1.87 μg/mL,检测限分别为1.34、1.06、0.98 μg/mL;精密度、稳定性、重复性试验的RSD均小于2.0%;加样回收率分别为98.01%~101.80%(RSD=1.38%,n=9)、98.38%~101.40%(RSD=1.26%,n=9)、98.06%~101.40%(RSD=1.28%,n=9);耐用性试验的RSD均小于2.0%。结论:该方法操作简便、准确,精密度、稳定性、重复性、耐用性好,可用于鼻炎灵片中绿原酸、黄芩苷和欧前胡素含量的同时测定。 |
ABSTRACT: | OBJECTIVE: To establish a method for simultaneous determination of chlorogenic acid, baicalin and imperatorin in Biyanling tablets. METHODS: HPLC method was adopted. The separation was performed on a Waters SunFire C18 column with mobile phase consisted of methanol-0.20% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm and column temperature was 30 ℃. The sample size was 20 μL. RESULTS: Linear ranges of chlorogenic acid, baicalin and imperatorin were 1.618-16.18 μg/mL, 1.624-16.24 μg/mL and 1.762-17.62 μg/mL (r were 0.999 9), respectively. The limits of quantitation were 2.67,2.03,1.87 μg/mL, and the limits of detection were 1.34, 1.06, 0.98 μg/mL, respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 98.01%-101.80%(RSD=1.38%,n=9), 98.38%-101.40%(RSD=1.26%,n=9)and 98.06%-101.40%(RSD=1.28%,n=9), respectively. RSDs of tolerance tests were all lower than 2.0%. CONCLUSIONS: The method is simple, accurate, precise, stable, reproducible and durable. It can be used for simultaneous determination of hlorogenic acid, baicalin and imperatorin in Biyanling tablets. |
期刊: | 2018年第29卷第12期 |
作者: | 邱红梅,杨林,何丹 |
AUTHORS: | QIU Hongmei,YANG Lin,HE Dan |
关键字: | 高效液相色谱法;鼻炎灵片;绿原酸;黄芩苷;欧前胡素;含量测定 |
KEYWORDS: | HPLC; Biyanling tablet; Chlorogenic acid; Baicalin; Imperatorin; Content determindtion |
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