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白耙齿菌固体发酵物对庆大霉素所致大鼠急性肾衰竭的改善作用研究
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| 篇名： | 白耙齿菌固体发酵物对庆大霉素所致大鼠急性肾衰竭的改善作用研究 |
| TITLE： | |
| 摘要： | 目的：研究白耙齿菌固体发酵物对庆大霉素（GM）所致大鼠急性肾衰竭的改善作用。方法：将50只大鼠随机分为空白对照组、模型组、阳性对照组（尿毒清颗粒，2 g/kg）和白耙齿菌固体发酵物低、高剂量组（2、4 g/kg），每组10只。除空白对照组外，其余各组大鼠均连续７ｄ腹腔注射GM（140 mg/kg）复制急性肾衰竭模型。给药组大鼠每天在造模2 h后灌胃给药1次，空白对照组和模型组在相应时间灌胃等量生理盐水；造模结束后，各组大鼠再连续给药7 d，每天给药1次。给药结束后，检测大鼠血肌酐（Scr）、血尿素氮（BUN）、尿肌酐（Ucr）和右肾组织中白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）含量，苏木精-伊红染色后观察大鼠左肾组织病理学变化。结果：与空白对照组比较，模型组大鼠Scr、BUN、Ucr和右肾组织中IL-6、TNF-α含量均显著增加（P＜0.01）；左肾组织发生肾小管上皮细胞明显水肿、变性、细胞核消失以及管腔内蛋白样沉积增多等病理学变化。与模型组比较，阳性对照组大鼠Ucr和白耙齿菌固体发酵物低剂量组大鼠BUN以及白耙齿菌固体发酵物高剂量组大鼠Scr、BUN、Ucr含量均显著减少（P＜0.05或P＜0.01）；同时给药组大鼠右肾组织中IL-6、TNF-α含量均显著减少（P＜0.05或P＜0.01）；左肾组织的炎症浸润减轻、肾小管和血管的增生受到抑制，病理学变化得到明显改善。结论：白耙齿菌固体发酵物对GM所致急性肾衰竭具有一定的改善作用。 |
| ABSTRACT： | OBJECTIVE： To study the improvement effects of Irpex lacteus solid fermentation products on gentamycin （GM）-induced acute renal failure in rats. METHODS： Fifty rats were randomly divided into blank control group， model group， positive control group （Niaoduqing granule， 2 g/kg） and Irpex lacteus solid fermentation products low-dose and high-dose groups （2， 4 g/kg）， with 10 rats in each group. Except for blank control group， other groups were given GM （140 mg/kg） intraperitoneally for consecutive 7 d to induce acute renal failure model. Administration group was given relevant medicine intragastrically 2 h after modeling， once a day. Blank control group and model group were given constant volume of normal saline at corresponding time. After modeling， each group was given relevant medicine for consecutive 7 d， once a day. After medication， the contents of Scr， BUN， Ucr and IL-6， TNF-α in right renal tissue were detected. The pathological changes of left renal tissue was observed by HE staining. RESULTS： Compared with blank control group， the contents of Scr， BUN， Ucr and IL-6， TNF-α in right renal tissue were increased significantly in model group （P＜0.01）. The pathological changes of left renal tissue were observed， such as obvious renal tubular epithelial cell edema， degeneration， nucleus disappearance and the increase of protein-like deposition in the lumen. Compared with model group， the content of Ucr in positive control group and the content of BUN in I. lacteus solid fermentation products low-dose group and the contents of Scr， BUN， Ucr in I. lacteus solid fermentation products high-dose group were decreased significantly （P＜0.05 or P＜0.01）； meanwhile， the contents of IL-6 and TNF-α in right renal tissue were decreased significantly in administration groups （P＜0.05 or P＜0.01）. The inflammatory infiltration of left renal tissue was relieved， and renal tubules and vessels hyperplasia were inhibited； the pathological changes were improved significantly. CONCLUSIONS： I. lacteus solid fermentation products could improve GM-induced acute renal failure to certain extent. |
| 期刊： | 2018年第29卷第13期 |
| 作者： | 李昂，黄静，刘旭，步洪石，刘馨晨，王淑敏 |
| AUTHORS： | LI Ang，HUANG Jing，LIU Xu，BU Hongshi，LIU Xinchen，WANG Shumin |
| 关键字： | 白耙齿菌；固体发酵物；急性肾衰竭；庆大霉素；大鼠 |
| KEYWORDS： | Irpex lacteus； Solid fermentation products； Acute renal failure； Gentamicin； Rats |
| 阅读数： | 216 次 |
| 本月下载数： | 4 次 |
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