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HPLC法同时测定消癌平片中通关藤苷A和通关藤苷I的含量
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| 篇名： | HPLC法同时测定消癌平片中通关藤苷A和通关藤苷I的含量 |
| TITLE： | |
| 摘要： | 目的：建立同时测定消癌平片中通关藤苷A和通关藤苷I含量的方法。方法：取消癌平片经三氯甲烷萃取后采用高效液相色谱（HPLC）法进样分析。色谱柱为Symmetry C18，流动相为乙腈-0.1％磷酸溶液（梯度洗脱），检测波长为223 nm，流速为1.0 mL/min，柱温为25 ℃，进样量为20 μL。采用上述条件测定3批样品中通关藤苷A和通关藤苷I的含量。结果：通关藤苷A、通关藤苷I的质量浓度检测线性范围分别为26.40～264.00 μg/mL（r＝0.999 4）、4.59～45.90 μg/mL（r＝0.999 3），检测限分别为0.26、0.12 μg/mL，定量限分别为0.79、0.36 μg/mL；精密度、稳定性（12 h）、重复性试验峰面积的RSD均＜2.0％（n＝6）；加样回收率分别为98.53％～99.28％（RSD为0.20％～0.51％，n＝9）、97.00％～98.89％（RSD为0.19％～1.03％，n＝9）。3批样品中通关藤苷A和通关藤苷I的含量分别为2.27～2.32、0.64～0.69 mg/g。结论：本试验建立的方法简便，结果准确可靠，适用于消癌平片中通关藤苷A和通关藤苷I含量的同时测定。 |
| ABSTRACT： | OBJECTIVE： To develop a method for simultaneous determination of tenacissoside A and tenacissoside I in Xiaoaiping tablets. METHODS： HPLC method was used to analyze Xiaoaiping tablets after extracted by trichlormethane. The determination was performed in Symmetry C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 223 nm， and the column temperature was 25 ℃. The sample size was 20 μL. The content of tenacissoside A and tenacissoside I in 3 batches of samples were determined by above condition. RESULTS： The linear range of tenacissoside A and tenacissoside I were 26.40-264.00 μg/mL （r＝0.999 4） and 4.59-45.90 μg/mL （r＝0.999 3）， respectively. The limits of detection were 0.26， 0.12 μg/mL， and the limits of quantification were 0.79， 0.36 μg/mL. RSDs of precision， stability （12 h） and repeatability tests were all lower than 2.0％（n＝6）. The recoveries were 98.53％-99.28％ （RSD＝0.20％-0.51％，n＝9） and 97.00％-98.89％ （RSD＝0.19％-1.03％，n＝9）. The contents of tenacissoside A and tenacissoside I in 3 batches of samples were 2.27-2.32 mg/g and 0.64-0.69 mg/g， respectively. CONCLUSIONS： The established method is accurate， simple and reliable， which is suitable for simultaneous determination of tenacissoside A and tenacissoside I in Xiaoaiping tablets. |
| 期刊： | 2018年第29卷第13期 |
| 作者： | 王祁民，马银玲，安静，张磊，孙利民，白万军，温艳虹，董占军 |
| AUTHORS： | WANG Qimin，MA Yinling，AN Jing，ZHANG Lei，SUN Limin，BAI Wanjun，WEN Yanhong，DONG Zhanjun |
| 关键字： | 高效液相色谱法； 消癌平片； 通关藤苷A； 通关藤苷I；含量测定 |
| KEYWORDS： | HPLC； Xiaoaiping tablets； Tenacissoside A； Tenacissoside I； Content determination |
| 阅读数： | 262 次 |
| 本月下载数： | 5 次 |
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