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大蒜素对模型小鼠的抗炎性痛作用及机制研究
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| 篇名： | 大蒜素对模型小鼠的抗炎性痛作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究大蒜素对模型小鼠的抗炎性痛作用及机制。方法：50只BALB/c小鼠随机分为模型组（等体积0.9％氯化钠溶液）、阿司匹林组（200 mg/kg）和大蒜素高、中、低剂量组（40、20、10 mg/kg），每组10只；均灌胃给药，每天1次，连续3 d。末次给药1 h后给予小鼠腹腔注射0.7％冰醋酸溶液（0.2 mL）建模，记录建模15 min内其扭体反应次数。分组与给药同上，末次给药1 h后给予小鼠左后足底皮下注射1.5％甲醛溶液（25 μL）建模，记录建模后0～10 min（第Ⅰ时相）和10～60 min（第Ⅱ时相）时小鼠累计舔足时间。分组与给药同上，末次给药1 h后给予小鼠右耳廓正反两面均匀涂抹二甲苯（30 μL）建模，建模2 h后测定小鼠耳廓肿胀度，以两耳质量差值为耳廓肿胀度，并计算耳廓肿胀抑制率。50只BALB/c小鼠随机分为空白对照组（等体积0.9％氯化钠溶液）、模型组（等体积0.9％氯化钠溶液）和大蒜素高、中、低剂量组（40、20、10 mg/kg），每组10只；均灌胃给药，每天1次，连续3 d。末次给药20 min后给予小鼠足趾皮下注射1％角叉菜胶0.9％氯化钠溶液（30 μL）建模，分别于建模前及建模1、3、5 h后测定小鼠足趾容积，以建模前后小鼠足趾容积差值为足趾肿胀度；测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛（MDA）含量以及总抗氧化能力（T-AOC）水平；采用Western blot法检测核转录因子κB（NF-κB）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）蛋白表达。结果：与模型组比较，阿司匹林组和大蒜素高、中剂量组小鼠扭体反应次数均显著减少；第Ⅰ时相时大蒜素高、中剂量组和第Ⅱ时相时阿司匹林组及大蒜素高、中剂量组小鼠累计舔足时间均显著缩短；阿司匹林组和大蒜素高剂量组小鼠耳廓肿胀度均显著降低；建模1、3 h后大蒜素高、中剂量组和建模5 h后大蒜素高剂量组小鼠足趾肿胀度均显著降低，差异均有统计学意义（P＜0.05或P＜0.01）。与空白对照组比较，模型组小鼠足趾肿胀组织中SOD、GSH-Px活性均显著减弱，MDA含量显著增加，T-AOC水平显著降低，NF-κB、TNF-α、IL-1β蛋白表达均显著增强，差异均有统计学意义（P＜0.01）；与模型组比较，大蒜素高剂量组小鼠足趾肿胀组织中SOD、GSH-Px活性均显著增强，MDA含量显著减少，且大蒜素高、中剂量组小鼠T-AOC水平均显著升高，同时大蒜素高剂量组小鼠足趾肿胀组织中NF-κB、TNF-α、IL-1β蛋白表达均显著减弱，差异均有统计学意义（P＜0.05或P＜0.01）。结论：大蒜素对模型小鼠炎性痛有一定的缓解作用，其机制与抗氧化应激反应和抑制NF-κB信号通路有关。 |
| ABSTRACT： | OBJECTIVE： To study the mechanism of anti-inflammatory pain effect of allicin in model mice. METHODS： Totally 50 BALB/c mice were randomly divided into model group （constant volume of 0.9％ sodium chloride solution），aspirin group （200 mg/kg），allicin high-dose，medium-dose and low-dose groups （40，20，10 mg/kg），with 10 mice in each group. They were given relevant medicine intragastrically，once a day，for consecutive 3 d. One hour after last medication，they were given intraperitoneal injection of 0.7％ glacial acetic acid solution （0.2 mL）； the times of writhing response were recorded with in 15 min after modeling. Grouping and administration were same as above； 1 h after last medication，mice were given subcutaneous injection of 1.5％ formaldehyde solution （25 μL） via left back toe； accumulative licking time of mice were recorded 0-10 min （time phaseⅠ） and 10-60 min （time phaseⅡ） after modeling. Grouping and administration were same as above； 1 h after last medication，mice were given xylene （30 μL） on both sides of right ear； the degree of auricular swelling of mice was determined 2 h after modeling. The mass difference of two ears was regarded as the degree of auricular swelling and then the inhibitory rate of swelling was calculated in mice. Totally 50 BALB/c mice were randomly divided into blank control group （constant volume of 0.9％ sodium chloride solution），model group （constant volume of 0.9％ sodium chloride solution） and allicin high-dose，medium-dose and low-dose groups （40，20，10 mg/kg），with 10 mice in each group. They were given relevant medicine intragastrically，once a day，for consecutive 3 d. Twenty minutes after last medication，mice were given subcutaneous injection of 1％ carrageenan 0.9％ sodium chloride solution （30 μL） via toe. Toe volume of mice was determined before modeling and 1，3，5 h after modeling. The difference of toe volume in mice was regarded as the degree of toe swelling. The activities of SOD and GSH-Px，contents of MDA，and level of T-AOC were determined. The expression levels of NF-κB，TNF-α and IL-1β protein were detected by Western blotting. RESULTS： Compared with model group，the times of writhing response were decreased significantly in aspirin group，allicin high-dose and medium-dose groups. The licking time of mice was shortened significantly in allicin high-dose and medium-dose group at time phaseⅠ， aspirin group，allicin high-dose and medium-dose at time phase Ⅱ. The degree of auricular swelling was decreased significantly in aspirin group and allicin high-dose group. The degree of paw swelling was decreased significantly in allicin high-dose and medium-dose groups 1，3 h after modeling， in allicin high-dose group 5 h after modeling，with statistical significance （P＜0.05 or P＜0.01）. Compared with blank control group，the SOD and GSH-Px activities，level of T-AOC in paw swelling tissue of mice were decreased significantly in model group，while MDA content，the protein expression of NF-κB，TNF-α and IL-1β were increased significantly，with statistical significance （P＜0.01）. Compared with model group，SOD and GSH-Px activities in paw swelling tissue of mice were increased， while MDA content were decreased of allicin high-dose group and the activity of T-AOC in allicin high-dose and medium-dose groups were increased significantly； the protein expression of NF-κB，TNF-α and IL-1β in paw swelling tissue of mice were decreased significantly in allicin high-dose，and medium-dose groups，with statistical significance （P＜0.05 or P＜0.01）. CONCLUSIONS： Allicin has certain anti-inflammatory pain effects，and its mechanism may be related to anti-oxidative stress and inhibition of NF-κB signaling pathway. |
| 期刊： | 2018年第29卷第18期 |
| 作者： | 任亮，任翔，刘金宝，朱吾元，杜钢军 |
| AUTHORS： | REN Liang，REN Xiang，LIU Jinbao，ZHU Wuyuan，DU Gangjun |
| 关键字： | 大蒜素；炎性痛；氧化应激；核转录因子κB；肿瘤坏死因子α；白细胞介素1β |
| KEYWORDS： | Allicin； Inflammatory pain； Oxidative stress； NF-κB； TNF-α； IL-1β |
| 阅读数： | 433 次 |
| 本月下载数： | 8 次 |
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