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槐花对照提取物的制备及槐花中4个黄酮类成分的含量测定
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| 篇名： | 槐花对照提取物的制备及槐花中4个黄酮类成分的含量测定 |
| TITLE： | |
| 摘要： | 目的：制备槐花对照提取物，并以其为对照测定槐花中4个黄酮类成分含量。方法：用水提法制备槐花对照提取物；采用高效液相色谱法测定其中芦丁、山柰酚-3-O-芸香糖苷、水仙苷和槲皮素的含量；色谱柱为Diamonsil C18（2），流动相为甲醇-1％冰乙酸水溶液（梯度洗脱），检测波长为360 nm，柱温为30 ℃，进样量为10 μL。以槐花对照提取物为对照测定槐花中4个成分的含量，并与以化学对照品为对照的测定结果进行比较。结果：在所制槐花对照提取物中，4个成分的含量占86.85％；芦丁、山柰酚-3-O-芸香糖苷、水仙苷和槲皮素的检测进样量线性范围分别为0.076 8～3.07、0.002 24～0.089 6、0.001 59～0.063 7、0.003 40～ 0.136 μg（r均≥0.999 5）；精密度、稳定性、重复性试验的RSD≤2.30％（n＝6），加样回收率分别为100.4％、96.99％、102.3％、100.9％（RSD分别为2.51％、1.58％、2.97％、3.20％，n＝6）。两种方法中4个成分的含量测定结果比较差异无统计学意义（P＞0.05）。 结论：以制备的槐花对照提取物为对照建立的测定方法操作简便、重现性好，并能同时测定槐花中4个成分的含量，可用于槐花饮片的质量控制。 |
| ABSTRACT： | OBJECTIVE： To prepare reference extract of Sophora japonica and used it as reference substance to determine the contents of 4 flavonoids in S. japonica. METHODS： The reference extract of S. japonica was prepared by water extraction. HPLC method was adopted to determine the contents of rutin， kaempferol-3-O-rutinoside， narcissoside and quercetin. The determination was performed on Diamonsil C18（2） column with mobile phase consisted of methanol-1％ acetic acid （gradient elution）. The detection wavelength was set at 360 nm， the column temperature was 30 ℃， and sample size was 10 μL. The contents of 4 components in S. japonica were determined by using reference extract of S. japonica as reference， and compared with the results with chemical control as reference. RESULTS： In prepared reference extract of S. japonica， the contents of 4 components were 86.85％. The linear range was 0.076 8-3.07 μg for rutin， 0.002 24-0.089 6 μg for kaempferol 3-O-rutinoside， 0.001 59-0.063 7 μg for narcissoside and 0.003 40-0.136 μg for quercetin （all r≥0.999 5）. RSD of precision， stability and reproducibility tests were all ≤2.30％ （n＝6）. The recoveries were 100.4％， 96.99％， 102.3％ and 100.9％（RSD were 2.51％， 1.58％， 2.97％， 3.20％， n＝6）， respectively. There was no statistical significance in the content determination of 4 components between 2 kinds of methods （P＞0.05）. CONCLUSIONS： Using prepared reference extract of S. japonica as reference， established method is simple and reproducible， and can be used for simultaneous determination of 4 components in S. japonica and quality control of S. japonica decoction piece. |
| 期刊： | 2018年第29卷第19期 |
| 作者： | 皮文霞，赵文望，蔡宝昌，陆兔林，郁红礼 |
| AUTHORS： | PI Wenxia，ZHAO Wenwang，CAI Baochang，LU Tulin，YU Hongli |
| 关键字： | 槐花；对照提取物；制备；高效液相色谱法；黄酮类成分；含量测定 |
| KEYWORDS： | Sophora japonica； Reference extract； Preparation； HPLC； Flavonoids； Content determination |
| 阅读数： | 448 次 |
| 本月下载数： | 7 次 |
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