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苦木水提物外用对变应性接触性皮炎模型小鼠的改善作用研究
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| 篇名： | 苦木水提物外用对变应性接触性皮炎模型小鼠的改善作用研究 |
| TITLE： | |
| 摘要： | 目的：考察苦木水提物外用对变应性接触性皮炎（ACD）模型小鼠的改善作用，为其进一步开发利用提供参考。方法：将48只小鼠随机分为空白对照组（生理盐水）、模型组（生理盐水）、氢化可的松组（阳性对照，约0.15 g/耳）和苦木水提物低、中、高剂量组（0.15、0.3、0.6 g/耳，以提取物计），每组8只。耳部涂抹给药，每天上午8点和下午4点分别给药1次，连续给药7 d。除空白对照组外，其余各组小鼠在第1、2天给药后于腹部涂抹含0.5％2，4-二硝基氟苯（DNFB）的丙酮-橄榄油溶液诱导ACD模型。第7天给药结束后，于造模组小鼠左耳涂抹0.25％DNFB的丙酮-橄榄油液诱发皮炎。24 h后，肉眼观察各组小鼠左耳组织变化并进行皮损评分，测定小鼠左、右耳厚度差和质量差，检测小鼠血清中免疫球蛋白E（IgE）和白细胞介素6（IL-6）含量，并在苏木精-伊红染色后于显微镜下观察小鼠耳组织病理形态学变化。结果：与空白对照组比较，模型组小鼠左耳皮损评分显著升高（P＜0.01），左、右耳厚度差和质量差以及血清中IgE、IL-6含量均显著增加（P＜0.01），显微镜下可见小鼠左耳组织出现淋巴细胞浸润、细胞间水肿、红细胞渗出等病理形态学变化。与模型组比较，氢化可的松组和苦木水提物高剂量组小鼠左耳皮损评分显著降低（P＜0.05或P＜0.01），各给药组小鼠左、右耳厚度差和质量差均显著减小（P＜0.01），氢化可的松组和苦木水提物中、高剂量组小鼠血清中IgE、IL-6含量均显著降低（P＜0.01），显微镜下可见各给药组小鼠左耳炎性病变均不同程度减轻。结论：苦木水提物外用对ACD模型小鼠具有较好的改善作用。 |
| ABSTRACT： | OBJECTIVE： To investigate the improvement effects of external application of water extract from Picrasma quassioides on allergic contact dermatitis （ACD） model mice， and to provide reference for its further development and utilization. METHODS： A total of 48 mice were randomly divided into blank control group （normal saline）， model group （normal saline）， hydrocortisone group （positive control， about 0.15 g/ear） and water extract from P. quassioides low-dose， medium-dose and high-dose groups （0.15， 0.3， 0.6 g/ear， calculated by extract）， with 8 mice in each group. The mice were given medicine on ear at 8 a.m. and 4 p.m. for 7 d continuously. Except for blank control group， other groups were given acetone-olive oil solution containing 0.5％ 2，4-dinitrofluorobenzene （DNFB） on the abdomen of mice to induce ACD model. After 7 d medication， modeling group was given acetone-olive oil of 0.25％ DNFB on left ear to induce dermatitis. 24 h later， the pathological changes of left ear tissue were observed by naked eye and skin lesion was scored. The thickness difference and weight difference of left and right ears in mice were measured. The serum contents of IL-6 and IgE were determined. The pathomorphology changes of ear tissue were observed by microscope after HE staining. RESULTS： Compared with blank control group， the skin lesion score of left ear was increased significantly in model group （P＜0.01）； the thickness difference and weight difference of left and right ears， serum contents of IgE and IL-6 were increased significantly （P＜0.01）. Pathological changes were observed in left tissue of mice by microscope， such as lymphocytic infiltration， intercellular edema， erythrocyte exudation. Compared with model group， skin lesion score of left ear in mice was decreased significantly in hydrocortisone group and water extract from P. quassioides high-dose group （P＜0.05 or P＜0.01）； the thickness difference and weight difference of left and right ears in mice were decreased significantly in all administration groups （P＜0.01）. The serum contents of IgE and IL-6 in mice were decreased significantly in hydrocortisone group， water extract from P. quassioides medium-dose and high-dose groups （P＜0.01）； the inflammatory lesions of left ear in mice were alleviated to varying degrees in different administration groups. CONCLUSIONS： External application of water extract from P. quassioides has a good improvement effect on ACD model mice. |
| 期刊： | 2019年第30卷第1期 |
| 作者： | 赵文娜，惠先，杨鑫，王清晖，张爱军，张抗怀 |
| AUTHORS： | ZHAO Wenna，HUI Xian，YANG Xin，WANG Qinghui，ZHANG Aijun，ZHANG Kanghuai |
| 关键字： | 苦木水提物；变应性接触性皮炎；免疫球蛋白E；白细胞介素6；小鼠；外用 |
| KEYWORDS： | Water extract from Picrasma quassioides；Allergic contact dermatitis； IgE；IL-6； Mice； External application |
| 阅读数： | 469 次 |
| 本月下载数： | 4 次 |
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