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蒙药新黑苏嘎乌日勒对脑中动脉阻塞-再灌注损伤模型大鼠神经功能损伤的改善作用及机制研究
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| 篇名： | 蒙药新黑苏嘎乌日勒对脑中动脉阻塞-再灌注损伤模型大鼠神经功能损伤的改善作用及机制研究 |
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| 摘要： | 目的：研究蒙药新黑苏嘎乌日勒对脑中动脉阻塞-再灌注损伤模型大鼠神经功能损伤的改善作用及机制。方法：将80只大鼠随机分为假手术组、模型组、新黑苏嘎乌日勒组（540 mg/kg）、化学药阳性对照组（尼莫地平片，16.12 mg/kg）、中药阳性对照组（银杏叶片，5.18 mg/kg），每组16只；除假手术组外，其余各组大鼠采用Zea-Longa线栓法复制脑中动脉阻塞-再灌注损伤模型；造模成功后，各给药组大鼠灌胃相应药物，假手术组和模型组灌胃等量蒸馏水，连续灌胃14 d。末次给药1 h后，对各组大鼠进行神经功能损伤评分；采用2，3，5-三苯基氯化四氮唑法计算各组大鼠脑梗死率、脑含水率及脑指数；苏木精-伊红染色观察各组大鼠脑组织海马区神经元病理学变化；免疫组化染色法检测各组大鼠脑前额叶脑源性神经营养因子（BDNF）和神经生长因子（NGF）蛋白表达水平。结果：与假手术组比较，模型组大鼠神经功能损伤评分、脑梗死率、脑含水率、脑指数和BDNF、NGF蛋白表达水平均显著升高（P＜0.01），脑组织海马区神经元细胞排列松散，形状不规则，部分胞体皱缩，胞浆内尼氏小体减少，核仁消失。与模型组比较，新黑苏嘎乌日勒组、化学药阳性对照组、中药阳性对照组大鼠神经功能损伤评分、脑梗死率、脑含水率、脑指数均显著降低（P＜0.05或P＜0.01），脑前额叶BDNF、NGF蛋白表达水平均显著升高（P＜0.05或P＜0.01），脑组织海马区神经元细胞排列整齐紧密，细胞核圆整，细胞质染色均匀。结论：蒙药新黑苏嘎乌日勒对脑中动脉阻塞-再灌注损伤模型大鼠神经功能损伤具有一定的改善作用，其作用机制可能与增加BDNF及NGF蛋白的表达有关。 |
| ABSTRACT： | OBJECTIVE： To study the improvement effect of neurological impairment and mechanism of Mongolian medicine New Hesugawurile on middle cerebral artery occlusion-reperfusion injury model rats. METHODS： Totally 80 rats were randomly divided into sham operation group， model group， New Hesugawurile group （540 mg/kg）， chemical drug positive control group （Nimodipine tablet， 16.12 mg/kg） and TCM positive control group （Ginkgo biloba leaves tablet， 5.18 mg/kg）， with 16 rats in each group. Except for sham operation group， middle cerebral artery occlusion-reperfusion injury model was estbalished by Zea-Longa suture method in other groups. After modeling， administration group was given relevant medicine intragastrically， while sham operation group and model group were given same volume of distilled water intragastrically， for consecutive 14 d. 1 h after last medication， neurological impairment score was conducted in each group. Cerebral infarction rate， cerebral water content and cerebral indexes were calculated by 2，3，5-triphenyltetrazolium chloride method. The pathological changes of cerebral hippocampal neurons of rats were observed by HE staining. The expression of brain-derived neurotrophic factor （BDNF） and nerve growth factor （NGF） protein in prefrontal lobe of rats were observed by immunohistochemical staining. RESULTS： Compared with sham operation group， the neurological impairment score， cerebral infarction rate， cerebral water content， cerebral index， the expression of BDNF and NGF protein were significantly rised in model group （P＜0.01）； the cerebral hippocampus neurons were loosely arranged， irregular in shape， while some of them shrank， nissl bodies in the cytoplasm decreased and the nucleoli disappeared. Compared with model group， the neurological impairment score， cerebral infarction rate， cerebral water content and cerebral indexes were decreased significantly in New Hesugawurile group， chemical drug positive control group and TCM positive control group （P＜0.05 or P＜0.01）， and the expression of BDNF and NGF protein in prefrontal lobe of rats were rised significantly （P＜0.05 or P＜0.01）. The cerebral hippocampus neurons were arranged tightly， with round nucleus and uniform cytoplasmic staining. CONCLUSIONS： Mongolian medicine New Hesugawurile can improve the neurological impairment of cerebral artery occlusion-reperfusion injury model rats， the mechanism of which may be associated with the expression up-regulation of BDNF and NGF protein. |
| 期刊： | 2019年第30卷第5期 |
| 作者： | 乌兰图雅，特格喜白音，玉兰，图雅 |
| AUTHORS： | Wulantuya，Tegexibaiyin，Yulan，Tuya |
| 关键字： | 蒙药；新黑苏嘎乌日勒；脑中动脉阻塞-再灌注损伤；大鼠；机制 |
| KEYWORDS： | Mongolian medicine； New Hesugawurile； Middle artery occlusion-reperfusion injury； Rats； Mechanism |
| 阅读数： | 442 次 |
| 本月下载数： | 2 次 |
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