参芍益气养血颗粒对小鼠实验性再生障碍性贫血的改善作用及骨髓抑制的防治作用研究-中国药房

参芍益气养血颗粒对小鼠实验性再生障碍性贫血的改善作用及骨髓抑制的防治作用研究

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篇名：	参芍益气养血颗粒对小鼠实验性再生障碍性贫血的改善作用及骨髓抑制的防治作用研究
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摘要：	目的：研究参芍益气养血颗粒对小鼠实验性再生障碍性贫血（AA）的改善作用及骨髓抑制的防治作用。方法：将72只小鼠随机分为空白组（生理盐水）、模型组（生理盐水）、阳性对照组（益血生胶囊，以内容物计为0.625 g/kg）和参芍益气养血颗粒低、中、高剂量组（以生药量计分别为1.26、7.56、15.12 g/kg），每组12只。除空白组外，其余各组小鼠均使用60Co-γ射线照射联合环磷酰胺（CTX）腹腔注射复制AA模型。造模成功后，每天灌胃给药1次，连续给药7 d。给药6 d后，将小鼠置于跑步机上进行力竭实验，测定其力竭距离和力竭时间。给药7 d后，测定各组小鼠外周血象[红细胞（RBC）、白细胞（WBC）、血小板（PLT）数目及血红蛋白（HGB）含量]变化；并通过血液涂片后测定其网织红细胞百分比（Ret），骨髓涂片后测定其骨髓有核细胞（BMNC）数目。另取90只小鼠，分组和给药剂量设置同前，每组15只。每天灌胃给药1次，连续给药12 d。给药3 d后，除空白组外，其余各组小鼠开始在给药同时腹腔注射5-氟尿嘧啶复制骨髓抑制模型。给药结束后，同法测定各组小鼠外周血象、Ret和BMNC数目。结果：在AA模型中，与空白组比较，模型组小鼠跑步力竭距离和力竭时间显著缩短（P＜0.01），同时外周血中RBC、WBC、PLT 数目和HGB含量以及Ret、BMNC数目显著降低（P＜0.05或P＜0.01）；与模型组比较，阳性对照组和参芍益气养血颗粒中、高剂量组小鼠的跑步力竭距离和力竭时间显著延长（P＜0.05或P＜0.01），阳性对照组和参芍益气养血颗粒高剂量组小鼠4项外周血象指标、Ret和BMNC数目以及参芍益气养血颗粒中剂量组小鼠Ret均显著升高（P＜0.05或P＜0.01）。在骨髓抑制模型中，与空白组比较，模型组小鼠外周血中WBC、RBC、PLT数目和Ret以及BMNC数目显著降低（P＜0.05或P＜0.01）；与模型组比较，阳性对照组和参芍益气养血颗粒高剂量组小鼠外周血中WBC、RBC、PLT数目和Ret、BMNC数目以及参芍益气养血颗粒中剂量组小鼠外周血中RBC数目、Ret和BMNC数目均显著升高（P＜0.05或P＜0.01）。结论：参芍益气养血颗粒对小鼠AA具有一定的改善作用，并且对小鼠骨髓抑制具有一定的防治作用。
ABSTRACT：	OBJECTIVE： To study the improvement effects of Shenshao yiqi yangxue granule on experimental aplastic anemia （AA） in mice and the preventive and therapeutic effects of it on myelosuppressive in mice. METHODS： Totally 72 mice were randomly divided into blank group （normal saline）， model group （normal saline）， positive control group （Yixuesheng capsule， 0.625 g/kg in content）， Shenshao yiqi yangxue granule low-dose， medium-dose and high-dose groups （1.26， 7.56， 15.12 g/kg by crude drug）， with 12 mice in each group. Except for blank group， other groups were given 60Co-γ irradiation combined with intraperitoneal injection of CTX to induce AA model. After modeling， they were given relevant medicine intragastrically once a day， for consecutive 7 d. After 6 days of administration， the mice were placed on a treadmill for exhaustion test， and the exhaustion distance and exhaustion time were measured. After 7 days of administration， the changes of peripheral blood （the number of RBC， WBC， PLT and content of HGB） were determined of mice in each group. The percentage of reticulocyte （Ret） was measured after blood smear， and the number of bone marrow nucleated cells （BMNC） was determined after bone marrow smear. Another 90 mice were collected， grouped and given relevant medicine as above， with 15 mice in each group. They were relevant medicine intragastrically once a day， for consecutive 12 d. After 3 days of administration， except for blank group， other groups were given 5-fluorouracil intraperitoneally to induce the bone marrow suppression model at the beginning of medication. After medication， peripheral hemogram， Ret and the number of BMNC were determined in each group. RESULTS： Compared with blank group， the exhaustion distance and exhaustion time of AA model group were significantly decreased （P＜0.01）， while the number of RBC， WBC， PLT and content of HGB， Ret and the number of BMNC were decreased significantly （P＜0.05 or P＜0.01）. Compared with model group， the exhaustion distance and exhaustion time of positive control group and Shenshao yiqi yangxue granule medium-dose and high-dose groups were increased significantly （P＜0.05 or P＜0.01）； the peripheral hemogram indicators， Ret and the number of BMNC were increased significantly in positive control group and Shenshao yiqi yangxue granule high-dose group， and Ret of Shenshao yiqi yangxue granule medium-dose group was increased significantly （P＜0.05 or P＜0.01）. In bone marrow suppression model， compared with blank group， the number of WBC， RBC and PLT， Ret， the number of BMNC were decreased significantly in model group （P＜0.05 or P＜0.01）. Compared with model group， the number of WBC， RBC and PLT， Ret and the number of BMNC in peripheral blood were increased significantly in positive control group and Shenshao yiqi yangxue granule high-dose group， and the number of RBC， Ret and the number of BMNC were increased significantly in Shenshao yiqi yangxue granule medium-dose group （P＜0.05 or P＜0.01）. CONCLUSIONS： Shenshao yiqi yangxue granule has certain improvement effects on AA in mice， and can prevent and treat myelosuppression in mice.
期刊：	2019年第30卷第11期
作者：	姚东风，许梦荣，马丽，邓颖颖，张恩户
AUTHORS：	YAO Dongfeng，XU Mengrong，MA Li，DENG Yingying，ZHANG Enhu
关键字：	参芍益气养血颗粒；再生障碍性贫血；骨髓抑制；外周血象；骨髓有核细胞；网织红细胞百分比；小鼠
KEYWORDS：	Shenshao yiqi yangxue granule； Aplastic anemia； Myelosuppression； Peripheral hemogram； Bone marrow nucleated cells； Percentage of reticulocyte； Mice
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