返回 
加入收藏
款冬花配方颗粒的质量标准研究
x
请在关注微信后，向客服人员索取文件
| 篇名： | 款冬花配方颗粒的质量标准研究 |
| TITLE： | |
| 摘要： | 目的：为建立款冬花配方颗粒的质量标准提供参考。方法：采用薄层色谱法（TLC）对款冬花配方颗粒进行定性鉴别；采用高效液相色谱法测定款冬花配方颗粒中款冬酮的含量，色谱柱为Thermo ODS Hypersil C18，流动相为甲醇-水（85 ∶ 15，V/V），流速为1.0 mL/min，检测波长为220 nm，柱温为25 ℃，进样量为20 μL。结果：款冬酮TLC图斑点清晰、分离度良好，阴性对照对测定无干扰。款冬酮检测质量浓度的线性范围为1.39～27.75 μg/mL（r＝0.999 9）；定量限、检测限分别为0.153 87、0.051 42 μg/mL；精密度、稳定性、重复性试验的RSD均小于2％；加样回收率为97.12％～103.96％（RSD＝2.60％，n＝6）。结论：该方法操作简单、准确、重复性好，可用于款冬花配方颗粒的质量控制。 |
| ABSTRACT： | OBJECTIVE： To provide reference for the establishment of quality standard of Tussilago farfara formula granules. METHODS： TLC method was used for qualitative identification of tussilagone in T. farfara formula granules. The content of tussilagone in T. farfara formula granule was determined by HPLC. The determination was performed on a Thermo ODS Hypersil C18 column with the mobile phase consisted of methanol-water （85 ∶ 15， V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 220 nm， the column temperature was 25 ℃. Sample size was 20 μL. RESULTS： TLC spots of tussilagone were clear and well-separated， without interference from negative control. The linear range of tussilagone was 1.39-27.75 μg/mL （r＝0.999 9）. The limits of quantification and detection were 0.153 87 and 0.051 42 μg/mL， respectively. RSDs of precision， stability and reproducibility tests were lower than 2％. The recoveries were 97.12％-103.96％ （RSD＝2.60％， n＝6）. CONCLUSIONS： The method is simple， accurate and reproducible， and suitable for quality control of T. farfara formula granules. |
| 期刊： | 2019年第30卷第14期 |
| 作者： | 许洪波，蔡兴航，毛晶晶，杨康，许怀礼 |
| AUTHORS： | XU Hongbo，CAI Xinghang，MAO Jingjing，YANG Kang，XU Huaili |
| 关键字： | 款冬花配方颗粒；质量标准；薄层色谱法；高效液相色谱法；款冬酮 |
| KEYWORDS： | Tussilago farfara formula granules； Quality standard； TLC； HPLC； Tussilagone |
| 阅读数： | 454 次 |
| 本月下载数： | 9 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
