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山慈菇中总黄酮的提取工艺优化
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| 篇名： | 山慈菇中总黄酮的提取工艺优化 |
| TITLE： | |
| 摘要： | 目的：建立山慈菇中总黄酮的含量测定方法，并优化其提取工艺。方法：采用紫外-可见分光光度法测定山慈菇中总黄酮的含量。以山慈菇中总黄酮含量为考察指标，以乙醇体积分数、料液比、提取时间、提取次数为考察因素，在单因素试验的基础上，采用L9（34）正交设计优化山慈菇中总黄酮的提取工艺。结果：芦丁检测质量浓度线性范围为8～48 μg/mL（r＝0.999 7）；定量限为0.54 μg/mL，检测限为0.18 μg/mL；精密度、稳定性、重复性试验的RSD均小于2％；加样回收率为99.61％～102.38％（RSD＝1.15％，n＝6）。最优提取工艺为70％乙醇、料液比1 ∶ 20（g/mL）、提取时间45 min、提取次数2次。在此条件下，山慈菇中总黄酮的平均含量为2.74 mg/g。结论：所建含量测定方法简单、准确；所得提取工艺稳定、可行。 |
| ABSTRACT： | OBJECTIVE： To establish a method for the content determination of total flavonoids from Typhonium divaricatum， and to optimize its extraction technology. METHODS： The content of total flavonoids in T. divaricatum was determined by UV spectrophotometry. Using the extraction amount of total flavonoids from T. divaricatum as index， the volume fraction of ethanol， the ratio of material to liquid， the extraction time and times as factors， the extraction technology of total flavonoids from T. divaricatum was optimized by L9（34） orthogonal design， based on the single factor test. RESULTS： The linear range of rutin were 8-48 μg/mL （r＝0.999 7）； the quantification limit was 0.54 μg/mL， and the detection limit was 0.18 μg/mL； RSDs of precision， stability and repeatability tests were all lower than 2％. The recoveries were 99.61％-102.38％（RSD＝1.15％， n＝6）. The optimal extraction technology was as follows： ethanol concentration of 70％， solid-liquid ratio of 1 ∶ 20 （g/mL）， extraction time of 45 min， extracting for 2 times. Under this condition， the average content of total flavonoids from T. divaricatum was 2.74 mg/g. CONCLUSIONS： Established method is simple and accurate； the extraction process is stable and feasible. |
| 期刊： | 2019年第30卷第14期 |
| 作者： | 王萌萌，谢典，周育芳，李晓静，徐步斌，周斌 |
| AUTHORS： | WANG Mengmeng，XIE Dian，ZHOU Yufang，LI Xiaojing，XU Bubin，ZHOU Bin |
| 关键字： | 山慈菇；总黄酮；芦丁；正交设计；提取工艺；紫外-可见分光光度法；含量测定 |
| KEYWORDS： | Typhonium divaricatum； Total flavonoids； Rutin； Orthogonal test； Extraction technology； UV spectrophotometry； Content determination |
| 阅读数： | 293 次 |
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