返回 
加入收藏
HPLC法同时测定半枝莲饮片中4种黄酮类成分的含量及主成分分析
x
请在关注微信后，向客服人员索取文件
| 篇名： | HPLC法同时测定半枝莲饮片中4种黄酮类成分的含量及主成分分析 |
| TITLE： | |
| 摘要： | 目的：建立同时测定半枝莲饮片中野黄芩苷、野黄芩素、木犀草素、芹菜素等4种黄酮类成分含量的方法，并进行主成分分析。方法：采用高效液相色谱法。色谱柱为Agilent ZOXDB-C18，流动相为甲醇-乙腈（80 ∶ 20，V/V）-1％醋酸水溶液（梯度洗脱），流速为1.0 mL/min，检测波长为335 nm，柱温为30 ℃，进样量为10 μL。采用SPSS 20.0、SIMCA-P 13.0软件进行主成分分析。结果：野黄芩苷、野黄芩素、木犀草素、芹菜素进样量的线性范围分别为0.131～1.446 μg（r＝0.999 0）、0.031～0.345 μg（r＝0.999 7）、0.005～0.055 μg（r＝0.999 2）、0.024～0.268 μg（r＝0.999 2）；定量限分别为1.178 8、0.602 9、0.744 1、1.079 1 ng，检测限分别为0.353 6、0.106 1、0.223 2、0.323 7 ng；精密度、稳定性、重复性试验的RSD均小于2％；加样回收率分别为99.38％～100.56％（RSD＝0.44％，n＝6）、91.01％～96.81％（RSD＝2.43％，n＝6）、91.44％～97.34％（RSD＝2.59％，n＝6）、96.21％～99.26％（RSD＝1.23％，n＝6）。主成分分析结果显示，主成分1和主成分2是影响样品质量评价的主要因子，2个主成分的累积方差贡献率为92.573％（＞80％）；S14-3样品综合评分最高，整体质量相对较好，S14-2、S14-1次之，这3批样品均系半枝莲种植基地药材加工产品，质量稳定。结论：本方法简单、快速，可用于同时测定半枝莲饮片中4种黄酮类成分的含量；主成分分析可为半枝莲饮片的质量控制提供参考。 |
| ABSTRACT： | OBJECTIVE： To establish the method for simultaneous determination of 4 kinds of flavones such as sutellarin， sutellarein， luteolin and apigenin in Scutellaria barbata decoction pieces， and to conduct principle component analysis. METHODS： HPLC method was adopted. The determination was performed on Agilent ZOXDB-C18 column with mobile phase consisted of methanol-acetonitrile （80 ∶ 20，V/V）-1％ acetic acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 335 nm， and column temperature was 30 ℃. The sample size was 10 μL. Principal component analysis was carried out by SPSS 20.0 and SIMCA-P 13.0 software. RESULTS： The linear ranges of sutellarin， sutellarein， luteolin and apigenin were 0.131-1.446 μg（r＝0.999 0）， 0.031-0.345 μg（r＝0.999 7）， 0.005-0.055 μg（r＝0.999 2）， 0.024-0.268 μg（r＝0.999 2）， respectively. The limits of quantitation were 1.178 8， 0.602 9， 0.744 1， 1.079 1 ng； the limits of detection were 0.353 6， 0.106 1， 0.223 2， 0.323 7 ng；RSDs of precision， stability and reproducibility tests were all lower than 2％. The recoveries were 99.38％-100.56％（RSD＝0.44％，n＝6）， 91.01％-96.81％（RSD＝2.43％， n＝6）， 91.44％-97.34％（RSD＝2.59％， n＝6）， 96.21％- 99.26％（RSD＝1.23％，n＝6）， respectively. By principal component analysis， principal component 1 and prinicipal component 2 were main influential factors of sample， quality accumulative variance contribution rate of them was 92.573％（＞80％）. The comprehensive score of sample S14-3 was the highest， and the overall quality was relatively good； samples S14-2， S14-3 were the second. These 3 batches of sample were processed and produced in S. barbata planting base with stable quality. CONCLUSIONS： Established method is simple and rapid， and can be used for simultaneous determination of 4 kinds of flavones in S. barbata decoction pieces. Principle component analysis can provide reference for the quality control of S. barbata decoction pieces. |
| 期刊： | 2019年第30卷第20期 |
| 作者： | 夏云岭，张振凌，张洪坤，林钦贤，梁伟龙，路丽 |
| AUTHORS： | XIA Yunling，ZHANG Zhenling，ZHANG Hongkun，LIN Qinxian，LIANG Weilong，LU Li |
| 关键字： | 半枝莲；野黄芩苷；野黄芩素；木犀草素；芹菜素；高效液相色谱法；含量测定；主成分分析 |
| KEYWORDS： | Scutellaria barbata； Sutellarin； Sutellarein； Luteolin； Apigenin； HPLC； Content determination； Principle component analysis |
| 阅读数： | 415 次 |
| 本月下载数： | 4 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
