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滋膵饮对2型糖尿病模型大鼠的降糖作用及机制研究
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| 篇名： | 滋膵饮对2型糖尿病模型大鼠的降糖作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究滋膵饮对2型糖尿病模型大鼠的降糖作用及其机制，为其临床应用提供理论基础。方法：将80只雄性大鼠高糖高脂饲料喂养4周后，单次腹腔注射链脲佐菌素35 mg/kg复制2型糖尿病大鼠模型，另取10只大鼠作为正常组。将糖尿病模型大鼠随机分为二甲双胍组（阳性对照，0.2 g/kg）、模型组（等体积蒸馏水）和滋膵饮高、中、低剂量组（14.0、7.0、3.5 g/kg），每组10只。连续灌胃给药2周后，采用全自动血糖仪测定各组大鼠空腹血糖值；采用酶联免疫吸附法测定各组大鼠血清胰岛素水平；采用逆转录-聚合酶链式反应（RT-PCR）检测各组大鼠胰腺组织中c-Jun氨基末端激酶（JNK）、蛋白激酶B（Akt）和胰岛素受体底物1（IRS-1）mRNA表达水平；Western blot法检测各组大鼠胰腺组织中JNK、Akt、IRS-1蛋白的磷酸化程度。结果：与正常组比较，模型组大鼠空腹血糖、胰腺组织中JNK mRNA的表达水平和JNK、IRS-1蛋白的磷酸化程度均显著升高（P＜0.05或P＜0.01），血清胰岛素含量、Akt mRNA、IRS-1 mRNA的表达水平和Akt蛋白的磷酸化程度均显著降低（P＜0.01）；与模型组比较，二甲双胍组和滋膵饮高、中剂量组大鼠空腹血糖均显著降低（P＜0.05），二甲双胍组和滋膵饮高剂量组大鼠血清胰岛素含量均显著升高（P＜0.01），二甲双胍组和滋膵饮各剂量组大鼠胰腺组织中JNK mRNA的表达水平均显著降低（P＜0.01）、Akt mRNA、IRS-1 mRNA的表达水平均显著升高（P＜0.01）；二甲双胍组和滋膵饮高剂量组大鼠的JNK、IRS-1蛋白的磷酸化程度均显著降低（P＜0.01）、Akt蛋白的磷酸化程度均显著升高（P＜0.01）。结论：滋膵饮具有明显的降糖作用，其作用机制与降低胰岛组织中JNK和IRS-1蛋白磷酸化程度和升高Akt蛋白磷酸化程度有关。 |
| ABSTRACT： | OBJECTIVE： To study the hypoglycemic effect and mechanism of Zicui yin on type Ⅱ diabetic rats， and to provide theoretic basis for clinical application. METHODS： Eighty male rats were fed with high fat/sugar diet for 4 weeks， and then given intraperitoneal injected with streptozotocin （35 mg/kg） to induce type 2 diabetic model. Other 10 rats were included in normal group. Model rats were randomly divided into metformin group （positive control， 0.2 g/kg）， model group （constant volume of distilled water）， Zicui yin high-dose， medium-dose， low-dose groups （14.0， 7.0， 3.5 g/kg）， with 10 rats in each group. After 2 weeks of continuous intragastic administration， the fasting blood glucose of rats in each group was measured by automatic blood glucose meter. The serum insulin level was determined by ELISA. mRNA expression of JNK， Akt and IRS-1 were detected by RT-PCR； The phosphorylation of JNK， Akt and IRS-1 proteins in the pancreatic tissue of rats in each group was detected by Western blot method. RESULTS： Compared with normal group， the fasting blood glucose， mRNA expression of JNK， The phosphorylation of JNK and IRS-1 proteins in the pancreatic tissue of the model group were significantly increased （P＜0.05 or P＜0.01）， while the serum insulin content， mRNA expression of Akt and IRS-1， the phosphorylation of Akt protein were significantly decreased （P＜0.01）. Compared with model group， the fasting blood glucose of rats in metformin group and Zicui yin high-dose and middle-dose groups decreased significantly （P＜0.05）， the serum insulin content of rats in both metformin group and Zicui yin high-dose group increased significantly （P＜0.01）， and mRNA expression of JNK in pancreatic tissue of rats in metformin group and Zicui yin groups decreased significantly （P＜0.01）， while mRNA expression of Akt and IRS-1 increased significantly （P＜0.01）. The phosphorylation of JNK and IRS-1 proteins in metformin group and Zicui yin high-dose group decreased significantly （P＜0.01）， while the phosphorylation of Akt protein were increased significantly （P＜0.01）. CONCLUSIONS： Zicui yin can significantly reduce blood glucose level， the mechanism of which may be related to decreasing the phosphorylation of JNK and IRS-1 proteins and increasing the phosphorylation of Akt proteins in pancreatic islets. |
| 期刊： | 2019年第30卷第21期 |
| 作者： | 刘欣欣，白茹，牛雯颖，王呈祥 |
| AUTHORS： | LIU Xinxin，BAI Ru，NIU Wenying，WANG Chengxiang |
| 关键字： | 滋膵饮；2型糖尿病；大鼠；胰岛素；c-Jun氨基末端激酶；蛋白激酶B；胰岛素受体底物1；血糖；作用机制 |
| KEYWORDS： | Zicui yin； Type Ⅱ diabetes； Rat； Insulin； JNK； Akt； IRS-1； Blood glucose； Mechanism |
| 阅读数： | 446 次 |
| 本月下载数： | 5 次 |
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