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人工蝉花对5-氟尿嘧啶诱发大鼠肠黏膜损伤的修复作用研究
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| 篇名： | 人工蝉花对5-氟尿嘧啶诱发大鼠肠黏膜损伤的修复作用研究 |
| TITLE： | |
| 摘要： | 目的：研究人工蝉花对5-氟尿嘧啶（5-FU）诱发大鼠肠黏膜损伤的修复作用。方法：将40只SD大鼠随机分为正常组（生理盐水）、模型组（生理盐水）和人工蝉花高、中、低剂量（3.5、1.75、0.875 g/kg）组，每组8只。除正常组外，其余各组大鼠每日腹腔注射30 mg/kg的5-FU（0.25 g/10 mL）1次，连续5 d；同时，各组大鼠每天灌胃相应药物/生理盐水1次，连续给药8 d。给药结束后，测定各组大鼠体质量变化，苏木精-伊红染色后观察各组大鼠小肠组织病理学变化，并检测各组大鼠生物屏障相关因子[血清中内毒素（ET）、D-乳酸（D-LA）]水平和免疫屏障相关因子[血清中肿瘤坏死因子α（TNF-α）、γ-干扰素（IFN-γ）、分泌性免疫球蛋白（sIgA）、白细胞介素15（IL-15）、集落刺激因子（G-CSF）和小肠组织中髓过氧化物酶（MPO）、丙二醛（MDA）]水平以及机械屏障相关因子（肠表面闭锁小带蛋白ZO-1和紧密连接蛋白Claudin-1）水平。结果：与正常组比较，模型组大鼠体质量显著减少（P＜0.01）；肠绒毛剥落明显，隐窝结构散乱，大量炎性细胞聚集，肠黏膜损伤严重；血清中D-LA、ET和TNF-α、IFN-γ、MPO、G-CSF水平以及小肠组织中MDA水平均显著升高（P＜0.01）；血清中sIgA、IL-15水平以及小肠组织中ZO-1、Claudin-1蛋白表达水平显著降低（P＜0.05或P＜0.01）。与模型组比较，人工蝉花高剂量组大鼠体质量均显著增加（P＜0.01）；各给药组大鼠小肠的病理变化均得到不同程度改善，其中人工蝉花高、中剂量组小肠形态接近正常组；各给药组大鼠血清中D-LA、ET和TNF-α、IFN-γ、MPO、G-CSF水平以及小肠组织中MDA水平均显著降低（P＜0.01）；各给药组大鼠血清中sIgA、IL-15水平以及人工蝉花高、中剂量组大鼠小肠组织中Claudin-1、ZO-1蛋白表达水平均显著升高（P＜0.01）。结论：人工蝉花可从机械屏障、免疫屏障、生物屏障等多个方面修复5-FU所致大鼠肠黏膜损伤。 |
| ABSTRACT： | OBJECTIVE： To study the repair effect of artificial Isaria cicadae on intestinal mucosal injury induced by 5-fluorouracil （5-FU） in rats. METHODS： Forty SD rats were randomly divided into normal group （normal saline）， model group （normal saline）， artificial I. cicadae high-dose， medium-dose and low-dose groups （3.5， 1.75， 0.875 g/kg）， with 8 rats in each group. Except for normal group， other groups were given intraperitoneal injection of 5-FU （0.25 g/10 mL） 30 mg/kg， once a day， for consecutive 5 days. At the same time， each group was given relevant medicine/normal saline intragastrically， once a day， for consecutive 8 d. After medication， body weight of rat was determined in each group. HE staining was used to observe the pathological change of small intestine. The levels of biobarrier-related factor [endotoxin （ET）， D-lactic acid （D-LA）]， immune barrier related factors （TNF-α， IFN-γ， sIgA， IL-15， G-CSF in serum and MPO， MDA in small intestine） and the levels of mechanical barrier related factors （connexin ZO-1 and Claudin-1） were detected. RESULTS： Compared with normal group， body weight of rats in model group was decreased significantly （P＜0.01）. Intestinal villus exfoliated obviously， the crypt structure was scattered， a large number of inflammatory cells gathered， and intestinal mucosa was seriously damaged. Serum levels of ET and D-LA， the levels of TNF-a， IFN-γ， MPO and G-CSF in serum， MDA level in small intestine were increased significantly （P＜0.01）. Serum levels of sIgA and IL-15 as well as the expressions of ZO-1 and Claudin-1 in small intestine were decreased significantly （P＜0.05 or P＜0.01）. Compared with model group， body weight of rats in artificial I. cicadae high-dose group was increased significantly （P＜0.01）. The pathological changes of the small intestine of rats in each administration group were improved to varying degrees. The intestine morphology of artificial I. cicadae high-dose and medium-dose groups was close to that of the normal group. The levels of and ET， D-LA， TNF-α， IFN-γ， MPO， G-CSF in serum and the level of MDA in intestinal were decreased significantly （P＜0.01）. Serum levels of sIgA and IL-15 in administration groups as well as the expressions of ZO-1 and Claudin-1 in intestinal tissue were increased significantly （P＜0.01）. CONCLUSIONS： Artificial I. cicadae can repair intestinal mucosal damage caused by 5-FU in respects of mechanical barrier， immune barrier， biological barrier. |
| 期刊： | 2019年第30卷第21期 |
| 作者： | 喻振，于瑞莲，邵佳蔚，杨哲，王惠 |
| AUTHORS： | YU Zhen，YU Ruilian，SHAO Jiawei，YANG Zhe，WANG Hui |
| 关键字： | 人工蝉花；肠黏膜损伤；5-氟尿嘧啶；大鼠；机械屏障；生物屏障；免疫屏障 |
| KEYWORDS： | Artificial Isaria cicadae；Intestinal mucosal injury； 5-Fluorouracil； Rat； Mechanical barrier；Biological?barrier； Immune barrier |
| 阅读数： | 339 次 |
| 本月下载数： | 4 次 |
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