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60Co-γ射线辐照诱导穿心莲M1代群体变异及聚类分析
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| 篇名： | 60Co-γ射线辐照诱导穿心莲M1代群体变异及聚类分析 |
| TITLE： | |
| 摘要： | 目的：研究60Co-γ射线辐照诱变对穿心莲植物学性状和品质性状的影响，筛选适宜的诱变辐照剂量。方法：采用不同辐照剂量（0、10、20、50、100、200、300 Gy）的60Co-γ射线辐照穿心莲种子，对辐照后诱变所得M1代穿心莲的种子发芽率、根长、出苗率、幼苗株高、叶片面积、鲜重、干重、叶片下表皮气孔数等植物学性状指标，以及穿心莲内酯含量、脱水穿心莲内酯含量、叶绿素含量、总超氧化物歧化酶（T-SOD酶）和铜锌超氧化物歧化酶（CuZn-SOD酶）等品质性状指标进行测定，计算变异系数（CV）；对出苗率进行线性回归分析，计算半致死剂量；对植物学性状和品质性状各指标与辐照剂量进行相关性分析；以组间联接法结合平方欧式距离对各辐照剂量组M1代穿心莲进行聚类分析。结果：不同辐照剂量对穿心莲植物学性状和品质性状的影响不同；按CV平均值大小对M1代穿心莲植物学性状指标排序为叶片面积＞鲜重＞干重＞株高＞根长＞气孔数＞出苗率＞发芽率，对不同辐照剂量排序为50 Gy＞200 Gy＞100 Gy＞20 Gy＞10 Gy＞300 Gy＞0 Gy；按CV平均值大小对M1代穿心莲品质性状指标排序为脱水穿心莲内酯含量＞穿心莲内酯含量＞叶绿素含量＞CuZn-SOD酶活性＞T-SOD酶活性，对不同辐照剂量排序为100 Gy＞50 Gy＞200 Gy＞20 Gy＞10 Gy＞300 Gy＞0 Gy；半致死剂量为195.10 Gy。根据植物学性状，可将7个剂量组M1代穿心莲分为4类；根据品质性状，可将7个剂量组M1代穿心莲分为3类。结论：穿心莲的适宜辐照诱变剂量范围为50～200 Gy。 |
| ABSTRACT： | OBJECTIVE： To study the effects of 60Co-γ radiation on the botanical traits and property of Andrographis paniculata， and to screen suitable irradiation dose. METHODS： The seeds of A. paniculata were irradiated by 60Co-γ rays with different irradiation doses（0，10，20，50，100，200，300 Gy）. The botanical traits indexes of A. paniculata as seed germination rate， root length， seedling rate， seedling height， leaf area， fresh weight， dry weight， stomata number of lower epidermis of leaf， and its property indexes as the contents of andrographolide， dehydrated andrographolide and chlorophyll， activity of T-SOD enzyme and CuZn-SOD enzyme， were determined after radiation. The coefficient of variation （CV） was calculated. The linear regression analysis was performed for seedling rate， and medial lethal dose was calculated. The correlation analysis was performed between the parameters of botanical trait and quality property with irradiation dose. Cluster analysis was conducted for M1 generation of A. paniculata in different irradiation dose groups by connection method combined with squared euclidean distance. RESULTS： Different irradiation doses showed different effects on botanical traits and property of A. paniculata. According to the average value of CV， the index of botanical traits was ranked as leaf area ＞ fresh weight ＞ dry weight ＞ plant height ＞ root length ＞ stomata number ＞ seedling rate ＞ germination rate； among different irradiation dose groups， the coefficient of variation was ranked as 50 Gy＞200 Gy＞100 Gy＞20 Gy＞10 Gy＞300 Gy＞0 Gy. According to the average value of CV， the index of property was ranked as dehydrated andrographolide content＞andrographolide content＞chlorophyll content＞CuZn-SOD enzyme activity＞T-SOD enzyme activity； among different irradiation dose groups， the coefficient of variation was ranked as 100 Gy＞50 Gy＞200 Gy＞20 Gy＞10 Gy＞300 Gy＞0 Gy. The medial lethal dose was 195.10 Gy. According to the botanical traits， M1 generation of A. paniculata of 7 dose groups could be divided into 4 types. According to the property， M1 generation of A. paniculata of 7 dose groups could be divided into 3 types. CONCLUSIONS： The suitable irradiation dose interval for irradiating A. paniculata is 50-200 Gy. |
| 期刊： | 2019年第30卷第24期 |
| 作者： | 苏雨苗，刘潇晗，杜勤，黎志鹏，石志棉，李娜，黄瑞华 |
| AUTHORS： | SU Yumiao，LIU Xiaohan，DU Qin，LI Zhipeng，SHI Zhimian，LI Na，HUANG Ruihua |
| 关键字： | 穿心莲；60Co-γ射线；辐照诱变育种；变异指数；聚类分析 |
| KEYWORDS： | Andrographis paniculata； 60Co-γ ray；Irradia- tion mutation breeding； Variation coefficient；Cluster analysis |
| 阅读数： | 383 次 |
| 本月下载数： | 3 次 |
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