目的：探究冠心病（CHD）患者与健康志愿者全基因组甲基化的差异，从表观遗传学角度初步探讨DNA甲基化与CHD的

相关性。方法：采用病例对照研究法，将受试者分为CHD组（CHD患者50 例）和健康对照组（Health 组，健康志愿者50 例），采用甲

基化DNA免疫共沉淀测序（MeDIP-Seq）法对两组受试者DNA进行测序，分析比较其全基因组甲基化的差异。结果：CHD组甲基

化峰（Peak）的数量高于Health 组，差异有统计学意义（P＜0.05）。Peak 主要分布在5’UTR、Intron 基因功能元件上。CHD 组

APQ1、SHB等基因启动子区域内的reads 数低于Health 组，其甲基化水平降低；而GRK5 基因及染色体chrX 上多个基因启动子区

域内的reads 数则高于Health 组，其甲基化水平增高，差异均有统计学意义（P＜0.01）。结论：CHD患者全基因组甲基化水平高于

健康志愿者，相关基因启动子甲基化水平的变化可能与CHD的发生有关。

OBJECTIVE：To explore the genome-wide methylation differences between coronary heart disease（CHD）patients

and healthy volunteers，and to investigate the relationsip of DNA methylation with CHD from epigenetics. METHODS：In case-control

study，subjects were divided into CHD group（50 cases）and health control group（50 cases）. DNA of 2 groups were sequenced with

methylated DNA immunoprecipitation sequencing technology. The genome-wide methylation differences were analyzed and compared

between 2 groups. RESULTS：The number of methylation peak in CHD group was higher than health group，with statistical signficance（

P＜0.05）. The methylation peak mainly distributed in 5’UTR，Intron functional elements. The number of reads in AQP1，SHB

and other gene promoters in CHD group were lower than health group，and its methylation level decreased. The number of reads in

GRK5 and serveal gene promoters on chrX in CHD group were higher than helath group，and its methylation level increased，with statistical

significance（P＜0.01）. CONCLUSIONS：The genome-wide methylation level of CHD patients are higher than those of healthy

volunteers. The occurence of CHD is possibly associated with the change of methylation level of related gene promoters.