目的：建立测定复方右旋糖酐70 滴眼液中右旋糖酐70 含量的方法。方法：采用高效液相色谱-示差折光检测法。色谱柱

为Phenomenex PolySep-GFC-P 4000，流动相为0.7％Na2SO4溶液，流速为0.5 ml/min，柱温为35 ℃，进样量为50 μl，示差折光检测

器检测池温度为40 ℃。结果：右旋糖酐70 检测质量浓度线性范围为0.1～5.0 mg/ml（r＝0.999 5）；精密度、稳定性、重复性试验的

RSD＜2％；平均回收率为97.45％～101.76％，RSD为0.57％（n＝9）。结论：本方法简便、快速、可靠，分离度高，分析时间短，可用

于复方右旋糖酐70滴眼液中右旋糖酐70 的含量测定。

OBJECTIVE：To establish a method for determination of dextran70 in Compound dextran70 eye drop. METHODS：

HPLC-RID was performed on the column of Phenomenex PolySep-GFC-P 4000 with the mobile phase of 0.7％ Na2SO4 solution.

The flow rate was 0.5 ml/min，column temperature was 35 ℃ and injection volume was 50 μl，and the inner temperature of refractive

index detector was 40 ℃. RESULTS：The linear range of dextran70 was 0.1-5.0 mg/ml（r＝0.999 5）；RSDs of precision，stability

and reproducibility tests were lower than 2％ ；average recovery was 97.45％ -101.76％（RSD＝0.57％ ，n＝9）. CONCLUSIONS：

The method is simple，fast and reliable with high separation and short analysis time，and can be used for the content determination

of dextran70 in Compound dextran70 eye drop.