目的：建立测定当归补血配方颗粒中黄芪甲苷含量的方法，并考察不同冲化条件对其含量的影响。方法：采用高效液相

色谱法。色谱柱为Agilent C18，流动相为乙腈-水（28 ∶72，V/V），流速为1.0 ml/min，柱温为25 ℃，进样量为10 μl。结果：黄芪甲苷检

测进样量线性范围为2.91～17.46 μg；精密度、稳定性的RSD＜2％、重复性试验的RSD＝3.63％，加样回收率95.76％～98.20％，

RSD为1.03％（n＝6）。较佳冲服方法为将两种颗粒混合后用80 ℃水冲化，保温5 min后服用。结论：本方法操作简便、重复性好，

可用于当归补血配方颗粒中黄芪甲苷含量的测定。

OBJECTIVE：To establish a method for content determination of astragaloside in Danggui buxue formula granule，

and investigate the influence of different conditions of dissolution on the content. METHODS：HPLC was performed on the column

of Aglient C18 with mobile phase of acetonitrile-water（28 ∶72，V/V）at flow rate of 1.0 ml/min，column temperature was 25 ℃，and

the injection volume was 10 μl. RESUITS：The linear range of astragaloside was 2.91-17.46 μg；RSDs of precision，stability were

lower than 2％ ，and reproducibility tests was 3.63％ ，recovery was 95.76％ -98.20％（RSD＝1.03％ ，n＝6）. The optimized use

method was mixing the two granules with 80 ℃ hot water and keeping heat preservation for 5 min successively before taken. CONCLUSIONS：

The method is simple and reproducible，and can be used for the content determination of astragaloside in Danggui buxue

formula granule.