目的：建立测定神香草中迷迭香酸和总多糖含量的方法。方法：采用高效液相色谱法测定神香草中迷迭香酸的含量。色

谱柱为Shim-pack VP-ODS，流动相为乙腈-0.5％甲酸（17 ∶83，V/V），流速为1.0 ml/min，检测波长为330 nm，柱温为30 ℃，进样量为

10 μl。采用紫外-可见分光光度法测定神香草中总多糖的含量，检测波长为620 nm。结果：迷迭香酸检测质量浓度线性范围为

10.86～54.30 μg/ml（r＝0.999 8）；精密度、稳定性、重复性的RSD＜1.0％；加样回收率为98.20％～101.79％，RSD 为1.68％（n＝

6）。总多糖检测质量浓度线性范围为1.996～15.97 μg/ml（r＝0.999 6）；精密度、稳定性、重复性试验的RSD＜2.0％；加样回收率为

96.62％～101.68％，RSD为1.88％（n＝6）。结论：该方法操作简便、重复性好，可用于神香草中迷迭香酸和总多糖含量的测定。

OBJECTIVE：To establish a method for content determination of rosmarinic acid and total polysaccharide in Hyssopus.

offcinalis. METHODS：HPLC was conducted to determine the content of rosmarinic acid in H. offcinalis. The column was

Shim-pack VP-ODS with mobile phase of acetonitrile-0.5％ formic（17 ∶ 83，V/V）at flow rate of 1.0 ml/min，detection wavelength

was 330 nm，column temperature was 30 ℃，and the injection volume was 10 μl. UV-visible spectrophotometry was conducted to

determine the content of total polysaccharide，detection wavelength was 620 nm. RESULTS：The linear range was 10.86-54.30 μg/ml

（r＝0.999 8）for rosmarinic acid and 1.996-15.97 μg/ml for total polysaccharide（r＝0.999 6）；RSDs of precision，stability and reproducibility

tests were lower than 1.0％ and 2.0％ ，respectively；recoveries were 98.20％ -101.79％（RSD＝1.68％ ，n＝6） and

96.62％-101.68％（RSD＝1.88％，n＝6）. CONCLUSIONS：The method is simple and reproducible，and can be used for the contents

determination of rosmarinic acid and total polysaccharide in H. offcinalis.