目的：建立鉴别重庆道地药材独活的生药学方法。方法：从原植物形态、性状特征、显微特征（横切面、粉末）、薄层色谱

（TLC）方面鉴别独活。结果：独活外形上根圆锥状，褐色，多分枝，具特异香气；主根横切面与侧根横切面显微特征无区别，初生木

质部为二原型，次生韧皮部较次生木质部发达，次生韧皮部中有丰富的油室。维管组织中含有大量薄壁组织细胞。TLC色谱中，

供试品与蛇床子素和二氢欧山芹醇当归酸酯以[石油醚（60～90 ℃）-乙酸乙酯（7 ∶3，V/V）为展开剂，紫外光灯下检视]对照品色谱

相应位置上显相同颜色的荧光斑点。结论：该方法操作简单、准确度高，可作为独活生药学鉴别的依据。

OBJECTIVE：To establish a method for the identification of Chongqing local medicinal material Heracleum hemsleyanum.

METHODS：The original plant morphology，morphological characteristics，microscopic characteristics （transverse section

and powder），were identified. RESULTS：H. hemsleyanum had cone shape root，brown，many-branched with special fragrance；the

microscopic characteristics of taproot a transverse showed no differences with lateral root transverse section，primary xylem was diarch，

secondary phloem was more developed than secondary xylem，secondary phloem was rich in the oil chamber. Vascular tissues

contained a large number of parenchyma cells. In TLC chromatrogram，the test sample showed the same color fluorescent spots withthe

substance reference of osthole and columbianadin in the corresponding locations. CONCLUSIONS：The established method is

simple with high accuracy，and can be used as the basis for identification of H. hemsleyanum.