目的：建立苍柏祛痛胶囊的质量标准。方法：采用薄层色谱（TLC）法对制剂中苍术、细辛、赤芍和黄柏进行鉴别；采用反

相高效液相色谱（RP-HPLC）法测定制剂中盐酸小檗碱的含量。色谱柱为Waters Symmetry-C18，流动相为乙腈-0.1％磷酸（梯度洗

脱），流速为1.0 ml/min，检测波长为265 nm，柱温为30 ℃，进样量为10 μl。结果：苍术、细辛、赤芍和黄柏的TLC图斑点清晰，分离

良好。盐酸小檗碱检测质量浓度线性范围为21.12～84.48 μg/ml（r＝0.999 6）；精密度、稳定性、重复性的RSD＜3％；加样回收率

为96.439％～100.950％，RSD为1.34％（n＝9）。结论：该方法操作简便、重复性好，可用于苍柏祛痛胶囊的质量标准评价。

OBJECTIVE：To establish the quality standard for Cangbai qutong capsule. METHODS：TLC was conducted to identify

the Rhizoma atractylodis，Asarum sieboldii，Paeoniae Radix Rubra and Cortex phellodendri；RP-HPLC was conducted to determine

the content of berberine hydrochloride. The column was Waters Symmetry-C18 with mobile phase of acetonitrile-0.1％ phosphoric

acid（gradient elution）at flow rate of 1.0 ml/min，the detection wavelength was 265 nm，column temperature was 30 ℃，

and the injection volume was 10 μl. RESULTS：TLC showed R. atractylodis，A. sieboldii，Paeoniae Radix Rubra and C. phellodendri

had clear spots and good separation. The linear range of berberine hydrochloride was 21.12-84.48 μg/ml（r＝0.999 6）；RSDs of precision，

stability and reproducibility tests were lower than 3％；recovery was 96.439％-100.950％（RSD＝1.34％，n＝9）. CONCLUSIONS：

The method is simple and reproducible，and can be used for the quality standards of Cangbai qutong capsule.