目的：建立利咽合剂的质量标准。方法：采用薄层色谱（TLC）法对制剂中的玄参、西青果、甘草进行鉴别；采用高效液相

色谱法测定制剂中绿原酸的含量。色谱柱为Agilent Eclipse Plus C18，流动相为甲醇-0.2％磷酸（18 ∶82，V/V），流速为1.0 ml/min，检

测波长为327 nm，柱温为30 ℃，进样量为20 μl。结果：玄参、西青果、甘草的TLC图斑点清晰，分离度好，阴性对照无干扰。绿原

酸检测进样量线性范围为0.16～1.6 μg（r＝0.999 9）；精密度、稳定性、重复性的RSD＜1.0％，加样回收率为96.9％～101.4％，RSD

为1.17％（n＝9）。结论：该方法操作简单、重复性好，可用于利咽合剂的质量控制。

OBJECTIVE：To establish the quality standard of Liyan mixture. METHODS：TLC was conducted to identify the

Scrophularia ningpoensis，Terminalia chebula and Glycyrrhiza uralensis；HPLC was conducted to determine the content of chlorogenic

acid. The column was Agilent Eclipse Plus C18 with mobile phase of methanol-0.2％ phosphoric acid（18 ∶ 82，V/V）at flow

rate of 1.0 ml/min，the detection wavelength was 327 nm，column temperature was 30 ℃ and the injection volume was 20 μl. RESULTS：

TLC showed S. ningpoensis，T. chebula and G. uralensis had clear spots and good separation. There was no interference

for negative control. The linear range of chlorogenic acid was 0.16-1.6 μg（r＝0.999 9）；RSDs of precision，stability and reproducibility

tests were lower than 1.0％，recovery was 96.9％-101.4％（RSD＝1.17％，n＝9）. CONCLUSIONS：The method is simple

and reproducible，and can be used for the quality control of Liyan mixture.