目的：建立测定乌芪舒筋通络片中乌头碱限量的方法。方法：采用薄层色谱（TLC）法对制剂中的乌头碱进行鉴定。薄层

板为硅胶G薄层板，展开剂为甲苯-乙酸乙酯-三氯甲烷-丙酮-氨水（20 ∶ 18 ∶ 3 ∶ 6 ∶ 1，V/V/V/V/V），显色剂为稀碘化铋钾试液和亚硝酸

钠乙醇试液。经耐用性和检测限考察，并优选薄层板、温度和湿度。结果：乌头碱的TLC图斑点清晰且阴性对照无干扰。本试验

耐用性良好；检测限为0.9 μg；可用薄层板为Merck HPTLC预制板、硅胶G薄层板和自制的以羧甲基纤维素钠为黏合剂的硅胶G

薄层板；温度为5～16 ℃，湿度为32％～72％。结论：本方法操作简便、重复性良好，可用于乌芪舒筋通络片中乌头碱限量的测定。

OBJECTIVE：To establish a method for the limit detection of aconitine in Wuqi shujin tongluo tablet. METHODS：

TLC was conducted to identify the aconitine；TLC plate was silica gel G plate，developing solvent was toluene-ethyl acetate-chloroform-

acetone-ammonia（20 ∶18 ∶3 ∶6 ∶1，V/V/V/V/V），chromogenic agent was bismuth potassium iodide test solution and sodium nitrite

ethanol test solution；and durability investigation and detection limit detection were used to optimize the TLC plate，temperature

and humidity. RESULTS：TLC showed the aconitine had clear spots and negative control without interference. The durability

was good；detection limit was 0.9 μ g；available TLC plate was Merck HPTLC prefabricated plate，silica gel G TLC plate

setf-made silica gel G TLC plate with adhesive of sodium carboxymethycellulose；temperature was 5-16 ℃ and humidity was

32％-72％. CONCLUSIONS：The method is simple and reproducibility，and can be used for the limit detection of aconitine in

Wuqi shujin tongluo tablet.