目的：建立测定银黄清火胶囊中绿原酸含量的方法。方法：采用高效液相色谱法。色谱柱为Acclaim120? C18，流动相为

乙腈-0.4％磷酸（12 ∶88，V/V），流速为1 ml/min，检测波长为327 nm，柱温为25 ℃，进样量为10 μl。结果：绿原酸检测进样量线性范

围为56～224 μg；精密度、稳定性、重复性的RSD＜3.0％；加样回收率为96.91％～104.76％，RSD为1.46％（n＝9）。结论：该方法

操作简便、重复性好，可用于银黄清火胶囊的质量控制。

OBJECTIVE：To establish a method to determine the content of chlorogenic acid in Yinhuang qinghuo capsule.

METHODS：HPLC was performed on the column of Acclaim120? C18 with mobile phase of acetonitrile -0.4％ phosphoric acid（12 ∶

88，V/V）at flow rate of 1 ml/min，the detection wavelength was 327 nm，column temperature was 25 ℃，and volume injection was

10 μl. RESULTS：The linear range of chlorogenic acid was 56-224 μg；RSDs of precision，stability and reproduciblity tests were

lower than 3.0％，recovery was 96.91％-104.76％（RSD＝1.46％，n＝9）. CONCLUSIONS：The method is simple and reproducible，

and can be used for the quality control of Yinhuang qinghuo capsule.