目的：建立测定白灵片中芍药苷含量的方法，并对测量结果进行不确定度评价。方法：采用高效液相色谱法。色谱柱为

Platisil C18，流动相为水-乙腈（87 ∶13，V/V），流速为1 ml/min，检测波长为230 nm，柱温为25 ℃，进样量为10 μl。对测定过程和步骤

进行量化分析，评价其不确定度。结果：芍药苷检测进样量线性范围为0.100 1～2.502 8 μg；精密度、稳定性、重复性试验的RSD＜

2.0％，加样回收率为98.7％～100.9％，RSD为0.86％（n＝6）。对照品称量、稀释过程和方法准确度对合成不确定度的贡献较大。

结论：该方法可用于白灵片中芍药苷的含量测定，不确定度评价提供了此方法的可信度。

OBJECTIVE：To establish a method to determinate the content of paeoniflorin in Bailing tablet，and to evaluate the

uncertainty of results. METHODS：HPLC was performed on the column of Platisil C18 with mobile phase of water - acetonitrile（87 ∶

13，V/V）at a flow rate of 1 ml/min，the detection wavelength was 230 nm，the column temperature was 25 ℃ and the injection

volume was 10 μ l. Quantitative analysis was conducted for the processes and procedures and the uncertainty was evaluated.

RESULTS：The linear range of paeoniflorin was 0.100 1-2.502 8 μg；RSDs of precision，stability and reproducibility tests were

lower than 2.0％，recovery rate was 98.7％-100.9％（RSD＝0.86％，n＝6）. Reference substance amount，elution produce and the

accuracy of method had great effect on the uncertainty. CONCLUSIONS：The established method can be used for the content

determination of paeoniflorin in Bailing tablet，and the uncertainty evaluation provides reliability for the method.