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壮通饮对心肌缺血再灌注后心律失常影响的实验研究
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| 篇名： | 壮通饮对心肌缺血再灌注后心律失常影响的实验研究 |
| TITLE： | Experimental Research on the Effects of Zhuangtongyin Decoction on Arrhythmia after Myocardial Ischemia-re- perfusion |
| 摘要： | 目的：观察壮通饮水提物对心肌缺血再灌注模型大鼠血液流变学和血脂水平的影响，初步探讨该方减少缺血再灌注后心律失常发生的分子生物学基础。方法：取SD大鼠75只，随机分为对照组、模型组和壮通饮水提物低、中、高剂量组（6.8、13.6、27.2g/kg），每组15只。通过结扎左心室回旋支末梢致缺血30min和再灌注60min，建立大鼠心肌缺血再灌注模型。术后第1天起，对照组、模型组大鼠均灌胃等体积水，壮通饮水提物各剂量组大鼠灌胃相应药液，每日1次，连续28d。分别在术后第7、14、21、28天检测大鼠心律失常发生情况并计算发生率。给药结束后，检测大鼠血液流变学指标和血清总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）含量；采用苏木精-伊红染色法观察大鼠心肌组织形态学；采用Westernblotting法检测大鼠心肌组织中凋亡相关因子FAS和FAS配体（FAS-L）的蛋白表达。结果：与对照组比较，模型组大鼠心律失常发生率显著升高，全血比黏度、血细胞比容（HCT）、体外形成血栓长度和质量、血小板凝聚率、红细胞滤过指数（IF）以及血清TC、TG、LDL-C含量均显著增加或升高，体内血栓的形成时间显著缩短，血清HDL-C含量显著降低，心肌组织中FAS和FAS-L蛋白相对表达量均显著升高（P＜0.05或P＜0.01）；大鼠心肌组织形态学呈明显病理改变。与模型组比较，壮通饮水提物各剂量组大鼠心律失常发生率呈明显下降趋势；除壮通饮水提物低剂量组大鼠的HCT降低不具有统计学意义（P＞0.05）外，其余给药组大鼠的全血比黏度、HCT、体外形成血栓的长度和质量、血小板凝聚率、IF值以及血清TC、TG、LDL-C含量均显著减少或降低，体内血栓的形成时间均显著延长，血清HDL-C含量均显著升高，心肌细胞中FAS和FAS-L蛋白相对表达量均显著降低（P＜0.05或P＜0.01）；大鼠心肌组织病理变化明显改善。结论：壮通饮可通过其活血化瘀作用改善心肌缺血再灌注模型大鼠的血液流变学指标，降低血清中TC、TG、LDL-C含量，提高HDL-C含量，并下调FAS和FAS-L蛋白表达，从而发挥抑制心肌细胞凋亡的作用，减少心律失常的发生。 |
| ABSTRACT： | OBJECTIVE：To investigate the e ffects of Zhuangtongyin water extract （ZTYWE）on hemorheology and blood lipid level of myocardial ischemia-reperfusion model rats ，and preliminarily explore its molecular biology foundation of reducing arrhythmia after myocardial ischemia-reperfusion. METHODS ：Totally 75 SD rats were divided into control group ，model group and ZTYWE low-dose ，middle-dose and high-dose groups. Myocardial ischemia-reperfusion model was established by ligating the left ventricle cyclotron endings for 30 min then reperfusing for 60 min. Since the first day after surgery ，control group and model group were intraperitoneally administrated with same volume normal saline ；ZTYWE low-dose ，middle-dose and high-dose groups were intraperitoneally administrated with medicine solution （6.8，13.6，27.2 g/kg），once a day ，for continuous 28 d. At the 7th， 14th，21st，28th day after surgery ，the incidence rate of arrhythmia of rats were detected. The hemorheology indicators and serum contents of TC ，TG，LDL-C，HDL-C were determined after the last administration. The morphology of myocardial tissue was observed by HE staining. The protein expressions of apoptosis-related factor FAS and FAS-L were detected by Western blotting assay. RESULTS ：Compared with control group ，the incidence rates of arrhythmia of rats in model group were significantly increased；the whole blood contrast viscosity ，hematocrit（HCT），in vitro forming length and quality of thrombosis ，platelet aggregation rate ，erythrocyte filtration index （IF），as well as the serum contents of TC ，TG and LDL-C were significantly increased；in vivo thrombus formation time as well as the serum content of HDL-C were significantly decreased ；the protein expression of FAS and FAS-L were significantly increased （P＜0.05 or P＜0.01）；obvious pathological changes were observed in myocardial tissue. Compared with model group ， the incidence rates of arrhythmia of rats in ZTYWE groups were in obvious reducing trend ； except for the HCT in 163.com low-dose ZTYWE group without significance ， the blood contrast viscosity ，HCT，in vitro forming length and quality of thrombosis ，platelet aggregation rate ，IF as well as the serum contents of TC ，TG and LDL-C were significantly decreased ；in vivo thrombus formation time as well as the serum contents of HDL-C were significantly increased ；the protein expressions of FAS and FAS-L were significantly decreased （P＜0.05 or P＜ 0.01）；the pathological changes of myocardial tissue were improved. CONCLUSIONS ：Through the promoting blood circulation and removing blood stasis effect ，Zhuangtongyin decoction can improve indexes of hemorheology index of ischemia-reperfusion model rats ，reduce the contents of TC ，TG and LDL-C ，enhace the contents of HDL-C ，down-regulate the protein expression of FAS and FAS-L ，so as to inhibit myocardial cells apoptosis and reduce arrhythmia. |
| 期刊： | 2020年第31卷第04期 |
| 作者： | 黄小珊，蓝凰齐，唐汉庆，张世田，庞路路，冯悦，黄岑汉 |
| AUTHORS： | HUANG Xiaoshan ，LAN Huangqi ，TANG Hanqing ，ZHANG Shitian ，PANG Lulu，FENG Yue，HUANG Cenhan |
| 关键字： | 壮通饮；心肌缺血再灌注；血液流变学；血脂；凋亡相关因子；冠心病；心律失常；大鼠 |
| KEYWORDS： | Zhuangtongyin decoction ； Myocardial ischemia-reperfusion ； Hemorheology； Blood lipid ；Apoptosis-related |
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