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苗药了哥王“汗渍法”炮制前后抗小鼠免疫性炎症的“量-效-毒”关系研究
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| 篇名： | 苗药了哥王“汗渍法”炮制前后抗小鼠免疫性炎症的“量-效-毒”关系研究 |
| TITLE： | Correlation Study of “Dose-effect-toxicity”of Miao Medicine Wikstroemia indica on Anti-immune Inflammation of Mice before and after Processed by “Sweat Soaking Method ” |
| 摘要： | 目的：探讨苗药了哥王炮制前后抗小鼠免疫性炎症的“量-效-毒”关系。方法：将小鼠分成空白组、模型组、了哥王生品醇提物1～6组、了哥王“汗渍法”炮制品醇提物1～6组（以下分别简称为“生品1～6组”“炮制品1～6组”，剂量均分别为0.13、0.20、0.26、0.52、1.04、2.6g/kg）和阳性组（环磷酰胺，36.4mg/kg），每组10只。除空白组外，其余各组小鼠均以1％2，4-二硝基氟苯-丙酮-麻油混合溶液复制迟发型超敏反应模型。造模后，空白组和模型组小鼠均灌胃等容1.0％羧甲基纤维素钠溶液，各给药组小鼠灌胃相应药液20mL/kg，每日1次，连续5d。末次给药后，记录各组小鼠耳肿胀度，并计算生品及炮制品的肿胀抑制率、半数有效剂量（ED50）及其95％置信区间（CI）；称定小鼠心、肝、脾、肺、肾的质量，并计算各脏器指数；采用酶联免疫吸附测定法、改良化学氧化法分别检测小鼠血清中炎症因子[肿瘤坏死因子α（TNF-α）、白细胞介素10（IL-10）]和肝肾功能指标[丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、总胆红素（TBIL）、尿素氮（BUN）、血肌酐（CREA）]水平。结果：与空白组比较，模型组小鼠耳肿胀度显著升高（P＜0.05）；与模型组比较，了哥王生药及炮制品醇提物各剂量组小鼠耳肿胀度均显著降低（P＜0.05），其肿胀抑制率均有随剂量增加而升高的趋势，两者抗小鼠迟发型超敏反应耳肿胀的ED50及95％CI分别为0.2396（0.1290，0.4452）g/kg、0.1473（0.0768，0.2827）g/kg。与空白组比较，模型组小鼠肝指数、血清TNF-α水平均显著升高，肺指数、血清IL-10水平均显著降低（P＜0.05）；与模型组比较，不同给药组小鼠肝指数（阳性组、生品3组和炮制品1～6组）、血清TNF-α水平（阳性组、生品1～3组及炮制品1～4组）均显著降低，肺指数（阳性组、生品3～6组及炮制品3～6组）、血清IL-10水平（生品1、2、4、5组以及炮制品2～6组）以及ALT、AST、BUN、CREA水平（生品4～6组）和TBIL水平（生品3～6组）均显著升高（P＜0.05）。结论：了哥王生品醇提物具有一定的抗免疫炎症活性，且对小鼠具有肝肾毒性，呈一定的“量-效-毒”关系；炮制品醇提物也具有一定的抗免疫炎症活性，但肝肾毒性弱于生品“。汗渍法”具有“存效减毒”的效果。 |
| ABSTRACT： | OBJECTIVE：To investigate the “dose-effect-toxicity”correlation of Miao medicine Wikstroemia indica before and after processed on anti-immnue inflammation of mice . METHODS ：Mice were divided into blank group ，model group ，ethanol extract of W. indica raw product groups 1-6，ethanol extract of W. indica processed product by “sweat soaking method ”groups 1-6 （hereinafter called “raw groups 1-6”“processed groups 1-6”for short ，drug dosage were 0.13，0.20，0.26，0.52，1.04，2.6 g/kg）， positive group （cyclophosphamide，36.4 mg/kg），with 10 mice in each group. Except for blank group ，other groups were given 1％ 2，4-dinitrofluorobenzene-acetone-sesame oil mixed solution to induce delayed type hypersensitivity model. After modeling， blank group and model group were given constant volume of 1.0％ CMC-Na solution intragastrically ，and administration groups were given relevant medicine 20 mL/kg intrag astrically，oncea day ，for consecutive 5 d. A fter last medication ，ear swelling degree of mice were recorded ；the inhibition rate of swelling degree， half effective dose （ED50） and 95％ confidence 158-02-32）； interval（CI）of raw and processed products were calculated ； the weight of heart ，liver，spleen，lung and kidney were measured and the indexes of organs were calculated ；ELISA 1161472062@qq.com and modified chemical oxidation method were used to determine the serum levels of inflammatory factors （TNF-α， IL-10） and liver and renal function indexes （ALT，AST， TBIL，BUN，CREA）. RESULTS：Compared with blank group ，the degree of ear swelling in the model group was significantly increased（P＜0.05）. Compared with model group ，ear swelling degree of mice were decreased significantly in different doses groups of ethanol extract of raw and processed products of W. indica （P＜0.05）. The inhibition rate of swelling increased with the increase of dose ，ED50 and 95％CI of delayed hypersensitivity ear swelling were 0.239 6（0.129 0，0.445 2）g/kg and 0.147 3（0.076 8，0.282 7）g/kg，respectively. Compared with blank group ，liver index and serum TNF-α level of mice were increased significantly in model group ，while lung index and serum IL- 10 level were decreased significantly （P＜0.05）. Compared with model group ，the levels of liver indexes （positive group ，raw group 3，processed groups 1-6）and serum TNF-α levels（positive group ，raw groups 1-3，processed groups 1-4） were decreased significantly in different administration groups ；while the levels of lung indexes （positive group ，raw groups 3-6 and processed groups 3-6），serum IL- 10 levels（raw groups 1，2，4 and 5，processed groups 2-6），ALT，AST，BUN and CREA levels （raw groups 4-6），TBIL levels （raw groups 3-6 ）were increased significantly （P＜ 0.05）. CONCLUSIONS ：The ethanol extract of raw product of W. indica has certain anti-inflammatory activity ，and has certain hepatorenal toxicity to mice ，with certain “dose-effect-toxity”correlation. The ethanol ectract of processed product of W. indica has certain anti-inflammatory activity too ，but its hepatorenal toxicity was lower than raw product. The “sweat soaking method ” possesses the function of “retaining efficiency and reducing toxicity ”for processing W. indica . |
| 期刊： | 2020年第31卷第06期 |
| 作者： | 郑传奇，冯果，李玮，周志容，许琴，李志平，易懂斌，李金徽 |
| AUTHORS： | ZHENG Chuanqi ，FENG Guo，LI Wei，ZHOU Zhirong ，XU Qin，LI Zhiping ，YI Dongbin ，LI Jinhui |
| 关键字： | 了哥王；汗渍法；免疫性炎症；剂量；药效；毒性 |
| KEYWORDS： | Wikstroemia indica ；Sweat soa king method ；Immune inflammation ；Dose；Effect；Toxicity |
| 阅读数： | 338 次 |
| 本月下载数： | 7 次 |
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