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不同生长年限三七花的指纹图谱建立及其人参皂苷的含量比较研究
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| 篇名： | 不同生长年限三七花的指纹图谱建立及其人参皂苷的含量比较研究 |
| TITLE： | Establishment of Fingerprints and Comparative Study on Ginsenoside Content of Panax notoginseng Flower with Different Growing Years |
| 摘要： | 目的：比较不同生长年限三七花的化学成分类别及人参皂苷含量的差异，探讨生长年限对三七花品质的影响。方法：收集2年生、3年生、4年生三七花样品各10批，采用高效液相色谱法（HPLC）进行测定。色谱柱为Shim-packGISTC18，流动相为乙腈-0.05％磷酸水溶液（梯度洗脱），柱温为30℃，流速为0.5mL/min，检测波长为203nm，进样量为20μL。采用《中药色谱指纹图谱相似度评价系统》绘制30批样品的指纹图谱并指认特征成分，同时进行相似度分析；采用SPSS22.0软件对样品进行聚类分析。采用上述HPLC法对不同生长年限三七花共30批样品中的人参皂苷Rb1、Rb2、Rb3、Rc进行含量测定；采用SPSS22.0软件进行质量控制分析。结果：所建指纹图谱的精密度、稳定性、重现性均良好；含量测定方法线性关系（R2＞0.999）、定量限、精密度、稳定性、重复性、准确度均良好。通过指纹图谱指认了不同生长年限三七花中人参皂苷Re、R1、Rb1、Rc、Rb2、Rb3等6个共有成分以及3年生三七花样品中的人参皂苷Rd；2年生、3年生、4年生三七花各10批样品指纹谱图在同一年限样品间比较的平均相似度分别为0.881、0.952、0.945，不同生长年限样品之间进行比较的相似度也均不低于0.817。30批三七花样品可聚为4个类别，第Ⅱ类均为4年生样品，第Ⅲ类均为3年生样品，第Ⅰ、Ⅳ类多为2年生样品以及少量3年生、4年生样品。2年生样品中4种人参皂苷成分含量及总含量的RSD为8.90％～21.43％，总皂苷含量为11.65％～17.76％；3年生样品中上述成分含量及总含量的RSD为6.45％～14.23％，总皂苷含量为15.74％～19.30％；4年生样品中上述成分含量及总含量的RSD为7.50％～18.86％，总皂苷含量为15.92％～20.16％。质量控制分析结果显示，2年生样品主要分布在Ⅱ、Ⅲ类区域，3年生、4年生样品主要分布在Ⅰ、Ⅱ类区域，人参皂苷成分的含量大小排序为Ⅰ＞Ⅱ＞Ⅲ。结论：不同生长年限三七花的化学成分类别大体接近，但仍存在一定差异，其中2年生样品中人参皂苷类成分含量较低、波动较大，整体品质略差；3年生、4年生样品中人参皂苷类成分含量更高、波动相对更小，整体品质较高且趋于稳定。 |
| ABSTRACT： | OBJECTIVE：To compare chemical composition types and ginsenoside content of Panax notoginseng flowers with different growing years ，and to explore the effect of growing year on the quality of P. notoginseng flowers. METHODS ：Each 10 batches of biennial，triennial and quadrennial P. notoginseng flower were collected and determined by HPLC. The determination was performed on Shim-pack GIST C 18 column with mobile phase consisted of acetonitrile- 0.05％ phosphoric acid solution （gradient elution ）at the flow rate of 0.5 mL/min. The column temperature was set at 30 ℃，and the detection wavelength was set at 203 nm. The sample size was 20 μL. Similarity Evaluation System of TCM Chromatogram Fingerprint was used to establish the fingerprint of 30 batches of samples ，identify the diagnostic components and analyze the similarity. Cluster analysis was conducted by using SPSS 22.0 software. The contents of ginsenoside Rb 1，Rb2，Rb3 and Rc in 30 batches of P. notoginseng flower with different growing years were determined by above HPLC . The quality control analysis was conducted by using SPSS 22.0 software. RESULTS：Established fingerprint showed good precision ，stability and reproducibility. There were good linear relationship （R2＞ 0.999），quantitative limit ，precision，stability，repeatability and accuracy of the content determination method . Six common components as ginsenoside Rb 1， Rb2， Rb3 and Rc were Δ 基金项目：云南省地方高校联合专项（No.KX182504Y） identified in P. notoginseng flower with different growing ＊助教，硕士。研究方向：中药资源开发 。电话：0876-2684947。 E-mail：wshuangzaiqiang@163.com years by fingerprint ；ginsenoside Rd was identified in triennial # 通信作者 ：研究员，硕士。研究方向 ：中药资源开发 。电话： P. notoginseng flower. The similarities of the fingerprints 0876-8883731。E-mail：gaomingju@163.com among 10 batches of biennial ，triennial and quadrennial P. 中国药房 2020年第31卷第8期 China Pharmacy 2020Vol. 31 No. 8 ·969· notoginseng flower were 0.881，0.952 and 0.945，respectively. The similarity among samples with different growing ye ars was more than 0.817. Thirty batches of P. notoginseng flower could be grouped into 4 categories，the category Ⅱ was quadrennial samples，the category Ⅲ was triennial samples ，while the categories Ⅰ and Ⅳ were mostly biennial samples and a small number of triennial and quadrennial samples. RSDs of 4 ginsenosides contents and their total contents in biennial samples were 8.90％-21.43％ and total saponin contents were 11.65％-17.76％，respectively. RSDs of 4 ginsenosides contents and their total contents in triennial samples were 6.45％-14.23％，and total saponin contents were 15.74％-19.30％. RSDs of 4 ginsenosides contents and their total contents in quadrennial samples were 7.50％-18.86％，and total saponin contents were 15.92％-20.16％. The results of quality control analysis showed that biennial samples mainly distributed in the areas of Ⅱ and Ⅲ ；triennial and quadrennial samples mainly distributed in the areas of Ⅰ and Ⅱ ；the order of ginsenosides content was Ⅰ ＞Ⅱ ＞Ⅲ. CONCLUSIONS：Chemical components of P. notoginseng flower with different growing years are generally close in types but there still a re some differences ，among which the content of ginsenosides in biennial samples is lower ，fluctuates more ，and the overall quality is slightly poor ；the content of ginsenosides in triennial and quadrennial samples is higher ，fluctuates less ，and the overall quality is higher and tends to be stable. |
| 期刊： | 2020年第31卷第08期 |
| 作者： | 黄再强，朱琳，高明菊，冯光泉，马小双，常征 |
| AUTHORS： | HUANG Zaiqiang，ZHU Lin，GAO Mingju，FENG Guangquan，MA Xiaoshuang，CHANG Zheng |
| 关键字： | 三七花；生长年限；高效液相色谱法；指纹图谱；人参皂苷；含量测定 |
| KEYWORDS： | Panax notoginseng flowers；Growing year ；HPLC；Fingerprint；Ginsenosides；Content determination |
| 阅读数： | 457 次 |
| 本月下载数： | 7 次 |
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