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干姜及其炮制品色差值与活性成分含量的相关性研究
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| 篇名： | 干姜及其炮制品色差值与活性成分含量的相关性研究 |
| TITLE： | Correlation Study of Color Difference Values and Active Constituent Contents in Crude and Processed Zingiber officinale |
| 摘要： | 目的：评价干姜及其炮制品色差值与活性成分含量的相关性。方法：采用高效液相色谱法测定6种活性成分的含量，采用色彩色差计测定干姜及其炮制品的色差值[明度（L＊）、红绿色轴分量（a＊）、黄蓝色轴分量（b＊）]。采用SPSS24.0软件对色差值与活性成分含量进行相关性分析。结果：姜酮、6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇和10-姜酚检测质量浓度的线性范围分别为2.65～105.90、10.15～406.00、4.87～194.80、5.28～211.20、6.14～245.70、7.02～280.80μg/mL（r均大于0.999）；定量限分别为7.46、13.68、14.37、16.62、17.03、17.99ng，检测限分别为2.24、4.11、4.31、4.99、5.11、5.40ng；精密度、稳定性、重复性试验的RSD均小于3％；平均加样回收率分别为101.34％、102.14％、101.22％，103.12％、103.74％、103.54％，103.06％、98.55％、99.43％，99.36％、103.51％、101.21％，100.85％、99.42％、99.60％，100.39％、97.69％、103.84％（RSD均小于3％，n＝3）；含量分别为0～0.66、0.06～7.57、0.03～1.45、0.29～3.47、0.15～2.85、0.04～2.83mg/g。色差值L＊、b＊与干姜不同炮制程度呈极显著负相关（P＜0.01），a＊与炮制程度呈正相关（P＜0.05）；干姜炮制前后L＊、b＊与姜酮含量呈极显著负相关，与其余5种成分呈极显著正相关（P＜0.01）；a＊与姜酮呈极显著正相关（P＜0.01），与其余5种成分无相关性（P＞0.05）；干姜及其炮制品与姜酮含量呈极显著正相关，与其余5种成分含量呈极显著负相关（P＜0.01）。结论：干姜及其炮制品的色差值与其活性成分含量相关，即随炮制程度的加重，其a＊增加，L＊、b＊降低；姜酮含量升高，6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇、10-姜酚含量降低。 |
| ABSTRACT： | OBJECTIVE：To evaluate the correlation between color difference values and active constituent contents of crude and processed Zingiber officinale . METHODS ：HPLC method was adopted to determint the content of 6 active constituents. The color difference values of crude and processed Z. officinale [lightness（L＊），red-green axis component （a＊），yellow-blue axis component（b＊）] were determined by chromatic aberration meter . SPSS 24.0 software was adopted for the correlation analysis between color difference values and active constituent contents. RESULTS ：The linear range of zingiberone ，6-gingerol， 8-gingerol， 6-shogaol， diacetoxy-6-gingerol and 10-gingerol were 2.65-105.90， 10.15-406.00， 4.87-194.80， 5.28-211.20， 6.14-245.70，7.02-280.80 μg/mL（r＞0.999）. The limits of quantification were 7.46，13.68，14.37，16.62，17.03，17.99 ng，and the limits of detection were 2.24，4.11，4.31，4.99，5.11，5.40 ng，respectively. RSDs of precision ，stability，and repeatability tests were all lower than 3％. The average recovery rates were 101.34％，102.14％，101.22％；103.12％，103.74％，103.54％；103.06％，properties critical for cell migration and invasion. induced EMT through downregulation of NF-κB-Snail sig- naling in breast cancer cells enchymal transition and activation of TLR 4/JNK signal - 98.55％，99.43％；99.36％，103.51％，101.21％；100.85％，99.42％，99.60％；100.39％，97.69％，103.84％（RSD were all lower than 3％，n＝3），respectively. The contents of them were 0-0.66，0.06-7.57，0.03-1.45，0.29-3.47，0.15-2.85，0.04-2.83 mg/g， respectively. L＊ and b＊ values were negative correlated with the processing degree of Z. officinale significantly（P＜0.01），a＊ showed a significantly positive correlation with the processing degree （P＜0.05）. L＊and b＊ values showed a significantly negative correlation with the content of zingiberone before and after processing ，but positively correlated with the other five components （P＜0.01）. a＊ showed a significantly positive correlation with the content of zingiberone ，but had no correlation with other five components（P＞0.05）. The crude and processed Z. officinale were positive correlated with the content of zingiberone ，negatively correlated with other five components （P＜0.01）. CONCLUSIONS ：There is a certain correlation between the color difference values of crude and processed Z. officinale and the contents of their active constituents. With the deepening of the processing ，a＊ values is increased ，L＊ values and b＊ values is decreased ；the content of zingiberone increases ，the contents of 6-gingerol， 8-gingerol，6-shogaol，diacetoxy-6-gingerol，10-gingerol reduce. |
| 期刊： | 2020年第31卷第10期 |
| 作者： | 林华坚，张梓豪，孟江，王淑美 |
| AUTHORS： | LIN Huajian ，ZHANG Zihao，MENG Jiang，WANG Shumei |
| 关键字： | 干姜；炮制品；高效液相色谱法；色彩色差计；活性成分；含量；色差值；相关性分析 |
| KEYWORDS： | Crude Zingiber officinale ；Processed product ；HPLC；Chromatric aberration meter ；Active constituent ；Content； |
| 阅读数： | 339 次 |
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