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基于综合评分和聚类分析的不同海拔、生长年限及干燥加工方法的黄连花薹的品质评价
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| 篇名： | 基于综合评分和聚类分析的不同海拔、生长年限及干燥加工方法的黄连花薹的品质评价 |
| TITLE： | Quality Evaluation of Inflorescence of Coptis chinensis with Different Altitude ，Growth Years and Drying Pro- cessing Methods Based on Comprehensive Score and Cluster Analysis |
| 摘要： | 目的：对不同海拔、生长年限及干燥加工方法的黄连花薹进行品质评价，为其开发利用及质量控制提供参考。方法：分别采用紫外-可见分光光度法和高效液相色谱法测定24批不同产地、不同生长年限及不同干燥加工方法的黄连花薹样品（S1～S24）中总黄酮和盐酸小檗碱的含量。以总黄酮和盐酸小檗碱含量为指标，并兼顾以干燥加工时间较短为优，分别对总黄酮含量、盐酸小檗碱含量、干燥时间标准化值赋予权重比例为30、40、30，计算其综合评分，对24批样品进行品质评价。以总黄酮和盐酸小檗碱含量为变量，应用SPSS19.0统计学软件对24批样品进行聚类分析。结果：海拔为1200m左右、生长年限在4年及以上的黄连花薹综合评分较高（84～94分），采用梯度干燥方法所得黄连花薹的综合评分普遍高于其他干燥加工方法所得样品。聚类分析结果显示，S1～S4、S9、S10、S13～S18聚为一类，其余12批聚为一类，与综合评分法分析结果基本一致。结论：不同海拔、生长年限及干燥加工方法对黄连花薹的品质均有一定影响，其中以海拔为1200m左右、生长年限在4年及以上、梯度干燥法加工的样品为优。 |
| ABSTRACT： | OBJECTIVE：To evaluate the quality of inflorescence of Coptis chinensis from different altitude with different growth years and drying processing methods ，and to provide reference for its utilization and quality control. METHODS ：The contents of total flavonoids and berberine hydrochloride in 24 batches of inflorescence of C. chinensis （S1-S24）from different altitude with different growth years and drying processing methods were determined by UV-Vis spectrophotometry and HPLC. Using the contents of total flavonoids and berberine hydrochloride as indexes ，and taking the short drying time as the best ，the weight ratios of total flavonoids content ，berberine hydrochloride content and standardized value of drying time were 30，40 and 30，respectively；comprehensive score was calculated ，then the quality of 24 batches of samples was evaluated. Using the contents of total flavonoids and berberine hydrochloride as variables ，systematic cluster analysis was performed for 24 batches of samples by using SPSS 19.0 statistical software. RESULTS ：For inflorescence of C. chinensis with altitude about 1 200 m and the growing years of 4 years and above ，the higher the comprehensive score （84-94 score）and the better the quality were. The comprehensive score of inflorescence of C. chinensis procesed by gradient drying method was generally higher than samples processed by other methods. Results of cluster analysis showed that S 1-S4，S9，S10，S13-S18 were clustered into one category ，and other 12 batches were clustered into one category ， which were basically consistent with the results of comprehensive scoring method. CONCLUSIONS：Different altitude ，different growth years and different drying processing methods have certain effects on the quality of inflorescence of C. chinensis ，among which the samples processed by gradient drying method with growth altitude of 1 200 m，growth years of 4 years and above are the best. |
| 期刊： | 2020年第31卷第10期 |
| 作者： | 伍利华，杨慧，杨俊莉，曾奇璐，刘涛，徐玉玲 |
| AUTHORS： | WU Lihua，YANG Hui，YANG Junli，ZENG Qilu，LIU Tao，XU Yuling |
| 关键字： | 黄连花薹；总黄酮；盐酸小檗碱；海拔；生长年限；干燥加工方法；综合评分法；聚类分析 |
| KEYWORDS： | Inflorescence of Coptis chinensis ；Total flavonoids ；Berberine hydrochloride ；Altitude；Growth years ；Drying |
| 阅读数： | 423 次 |
| 本月下载数： | 3 次 |
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