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蛤蚧生品及不同炮制品对腺嘌呤致肾阳虚模型小鼠的改善作用比较
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| 篇名： | 蛤蚧生品及不同炮制品对腺嘌呤致肾阳虚模型小鼠的改善作用比较 |
| TITLE： | Comparative Study on the Effect of Raw Product and Different Processed Products of Gekko gecko on Kidney- yang Deficiency Model Mice Induced by Adenine |
| 摘要： | 目的：比较蛤蚧生品及不同炮制品对腺嘌呤致肾阳虚模型小鼠的改善作用。方法：将100只小鼠随机分为空白组（n＝10）和造模组（n＝90）。造模组小鼠灌胃腺嘌呤（50mg/kg）10d以复制肾阳虚模型，空白组小鼠灌胃生理盐水（0.2mL/10g）。将造模成功的70只小鼠按体质量和肾阳虚症状程度随机分为模型组、阳性组（金匮肾气丸，6.4g/kg）、蛤蚧生品组（1.2g/kg）、酒蛤蚧组（1.2g/kg）和油酥蛤蚧组（1.2g/kg），每组14只。空白组和模型组小鼠灌胃等体积生理盐水，各给药组小鼠灌胃相应药物混悬液，20mL/kg，每天给药1次，连续给药14d。实验期间，观察各组小鼠的症状和体征情况。末次给药结束后，测定各组小鼠的肾指数、睾丸指数以及血清中睾酮、皮质醇、尿素氮、肌酐水平，并采用苏木精-伊红染色法观察肾组织病理学变化。结果：与空白组比较，模型组小鼠出现体质量减轻、蜷缩拱背、畏寒肢冷、体毛稀疏等肾阳虚表现；肾指数以及血清中尿素氮、肌酐水平显著升高（P＜0.01）；肾组织可见肾小管上皮细胞排列不整齐、细胞核淡染、细胞质水肿等病理损伤。与模型组比较，蛤蚧生品和各炮制品组小鼠的肾阳虚表现均有不同程度改善（尤以酒蛤蚧组小鼠改善最为明显），血清中肌酐、尿素氮水平均显著降低（P＜0.05或P＜0.01），肾组织病理损伤有不同程度的减轻；此外，蛤蚧各炮制品组小鼠体质量显著升高（P＜0.01），蛤蚧生品组和各炮制品组小鼠肾指数显著降低（P＜0.05或P＜0.01）。与蛤蚧生品组比较，酒蛤蚧组小鼠肾指数以及血清中尿素氮、肌酐水平均显著降低（P＜0.01），油酥蛤蚧组小鼠血清中肌酐水平显著降低（P＜0.05或P＜0.01）。结论：蛤蚧生品、酒蛤蚧、油酥蛤蚧对腺嘌呤所致肾阳虚模型小鼠均具有一定的改善作用，其中以酒蛤蚧的作用最为明显。 |
| ABSTRACT： | OBJECTIVE：To compare the effects of raw pro duct and different processed products of Gekko gecko on kidney-yang deficiency model mice induced by adenine. METHODS ：Totally 100 mice were randomly divided into blank group （n＝10）and modeling group （n＝90）. Modeling group was given adenine （50 mg/kg）intragastrically for 10 days to induce kidney-yang deficiency model ；blank group was given normal saline （0.2 mL/10 g）intragastrically. After modeling ，70 mice were randomly divided into model group ，positive group （Jinkui shenqi pill ，6.4 g/kg），G. gecko crude product group （1.2 g/kg）， wine-processed G. gecko group（1.2 g/kg）and oil-processed G. gecko group（1.2 g/kg）according to body weight and symptoms of kidney-yang deficiency ，with 14 mice in each group. Blank group and model group were given constant volume of normal saline intragastrically；administration groups were given relevant medicine intragastrically （0.2 mL/10 g），once a day ，for consecutive 14 d. During the experiment ，the symptoms and signs of mice in each group were observed. After last medication ，renal index ，testis index and serum levels of T ，CORT，BUN and Cr were measured ；HE staining method was used to observe the pathological changes of renal tissue of mice in each group. RESULTS ：Compared with blank group ，the mice in the model group suffered from performance of kidney-yang deficiency ，such as weight loss ，crouch and arch back ，chills and cold limbs ，and sparse body hair ， while renal index and serum levels of BUN and Cr were increased significantly （P＜0.01）. In renal tissue ，there were BA28117 pathological damages such as irregular arrangement of renal KY2016YB211tubular epithelial cells ，light staining of nucleus and edema of cytoplasm. Compared with model group ， performance of kidney-yang deficiency was improved to different extents in G. gecko crude product group and processed product groups（especially in wine-processed G. gecko group）；serum levelsof BUN and Cr were decreased significantly （P＜0.05 or P＜0.01）；pathological damage of renal tissue was alleviated in different degrees. In addition ，body weight of mice was increased significantly in G. gecko processed products groups （P＜0.01），and renal indexes of mice were decreased significantly in G. gecko crude product group and processed products groups （P＜0.05 or P＜0.01）. Compared with G. gecko crude product group ，renal index ，serum levels of BUN and Cr were significantly decreased in wine-processed G. gecko group（P＜0.01），and serum level of Cr was significantly decreased in oil-processed G. gecko group（P＜ 0.05）. CONCLUSIONS ：G. gecko crude product ，wine-processed G. gecko and oil-processed G. gecko all show a certain improvement effect on kidney-yang deficiency mice induced by adenine ，especially wine-processed G. gecko . |
| 期刊： | 2020年第31卷第13期 |
| 作者： | 黄馨慧，王晓珊，刘舒凌，吴燕春，周蓓，张兵，熊桂玉 |
| AUTHORS： | HUANG Xinhui ，WANG Xiaoshan ，LIU Shuling ，WU Yanchun ，ZHOU Bei，ZHANG Bing，XIONG Guiyu |
| 关键字： | 蛤蚧；生品；炮制品；肾阳虚；腺嘌呤；小鼠 |
| KEYWORDS： | Gekko gecko ；Raw product ；Processed product ；Kidney-yang deficiency ；Adenine；Mice |
| 阅读数： | 358 次 |
| 本月下载数： | 5 次 |
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