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沙苑子总黄酮对雄性生殖功能障碍模型大鼠的改善作用及机制研究
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| 篇名： | 沙苑子总黄酮对雄性生殖功能障碍模型大鼠的改善作用及机制研究 |
| TITLE： | Study on Improvement Effects and Mechanism of Astragalus complanatus Total Flavonoids on Reproductive Dysfunction Model Male Rats |
| 摘要： | 目的：研究沙苑子总黄酮（TFA）对腺嘌呤诱导的雄性生殖功能障碍模型大鼠的改善作用及机制。方法：将雄性SD大鼠分为空白组、模型组、阳性组（五子衍宗丸，2g/kg）和TFA低、中、高剂量组（0.05、0.1、0.2g/kg），每组12只。各组雄性大鼠每日早上9：00灌胃相应药物，并于同日下午3：00灌胃腺嘌呤混悬液以复制生殖功能障碍模型（空白组除外），连续给药35d。给药结束后，将各组雄性大鼠与雌性大鼠按1∶1合笼交配7d后取出，检测致雌鼠受孕率；采用酶联免疫吸附法检测雄性大鼠血清中睾酮（T）、雌二醇（E2）、促卵泡激素（FSH）、促黄体激素（LH）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、丙二醛（MDA）、肌酐（Scr）、尿素氮（BUN）水平；摘取雄性大鼠睾丸、附睾、精囊腺、胸腺、肾，并计算其脏器指数；采用苏木精-伊红（HE）染色法观察雄性大鼠睾丸、附睾及肾组织的病理学变化；采用免疫组织化学染色检测雄性大鼠肾组织中B细胞淋巴瘤/白血病2（Bcl-2）、Bcl-2相关X蛋白（Bax）、胱天蛋白酶3（Caspase-3）、Caspase-9的蛋白表达水平。结果：与空白组比较，模型组雄性大鼠致雌鼠受孕率，睾丸、附睾、精囊腺、胸腺指数，血清中T、E2、SOD、GSH-Px水平，肾组织中Bcl-2蛋白表达水平均显著降低（P＜0.01）；肾指数和血清中FSH、LH、MDA、Scr、BUN水平，肾组织中Bax、Caspase-3、Caspase-9蛋白表达水平均显著升高（P＜0.01）；睾丸组织中生精小管直径变细、间距较宽，生精细胞排列紊乱；附睾管管壁增厚、排列疏松、间质增大，管腔增生萎缩、腔内精子聚集成团；肾组织中肾间质纤维增生，肾小管内可见大量黄色结晶。与模型组比较，阳性组和TFA中、高剂量组雄性大鼠致雌鼠受孕率，睾丸、附睾、精囊腺、胸腺指数，血清中T、E2、SOD、GSH-Px水平，肾组织中Bcl-2蛋白表达水平均显著升高（P＜0.05或P＜0.01），肾指数和血清中FSH、LH、MDA、Scr、BUN水平，肾组织中Bax、Caspase-3、Caspase-9蛋白表达水平均显著降低（P＜0.05或P＜0.01）；TFA低剂量组上述指标变化趋势相同，且大部分有显著性差异（P＜0.05或P＜0.01）；各给药组雄性大鼠睾丸、附睾、肾组织病理学变化均明显减轻。结论：TFA对腺嘌呤诱导的雄性大鼠的生殖障碍具有显著改善作用，其机制可能与降低性激素FSH、LH水平和下调肾组织中凋亡相关蛋白表达，升高性激素T、E2水平和改善机体氧化应激水平有关。 |
| ABSTRACT： | OBJECTIVE： To stu dy the improvement effects of Astragalus complanatus total flavonoids （TFA） on adenine-induced reproductive dysfunction model male rats and its mechanism. METHODS ：Male SD rats were divided into blank group，model group ，positive group （Wuzhi yanzong pill ，2 g/kg），TFA high-dose，medium-dose and low-dose groups （0.05， 0.1，0.2 g/kg），with 12 rats in each group. Male rats in each group were given the corresponding drugs intragastrically at 9：00 a.m. every day ，while the model of reproductive dysfunction was reproduced by intragastric administration of adenine suspension （except for blank group ）at 3：00 p.m. on the same day ，for consecutive 35 days. After medication ，male rats in each group were mated with female rats by ratio of 1∶1 in cages ；7 days later ，inducing-pregnancy rate of male rats were measured. ELISA assay was used to detect the serum levels of T ，E2，FSH，LH，GSH-Px，SOD，MDA，Scr and BUN in male rats ；the testis ， epididymis，seminal vesicle ，thymus and kidney of male rats were extracted and the organ index was calculated ；HE staining was used to observe the pathological changes of testis ，epididymis and kidney ；the protein expression of Bcl- 2，Bax，Caspase-3 and Caspase-9 in the renal tissue of male rats were detected by immunohistochemistry. RESULTS ：Compared with blank group ，the inducing-pregnancy rate of male rats ，the testis ，epididymis，seminal vesicle gland ，thymus indexes ，the serum levels of T ，E2， SOD and GSH-Px ，the protein expression levels of Bcl- 2 in renal tissue were reduced significantly in model group （P＜0.01）；the renal index ，the serum levels of FSH ，LH，MDA，Scr and BUN ，the protein expression levels of Bax ，Caspase-3 and Caspase- 9 in renal tissue were increased significantly （P＜0.01）；the diameter of seminiferous tubules in testis became thinner ，the spacing was wider ，and the arrangement of seminiferous cells was disordered ；the wall of epididymis was thickened ，the arrangement was loose，the stroma was enlarged ，the lumen was proliferated and atrophied ，and the sperm in the lumen wass aggregated ；in renal tissue，interstitial fibers was proliferated ，and a large number of yellow crystals were seen in renal tubules. Compared with model group，the inducing-pregnancy rate，the testis ，epididymis，seminal vesicle gland ，thymus indexes ，the serum levels of T ，E2， SOD and GSH-Px ，the protein expression levels of Bcl- 2 in renal tissue of male rats were increased significantly in the positive group，TFA medium-dose and high-dose groups （P＜0.05 or P＜0.01），while the renal index ，the serum levels of FSH ，LH， MDA，Scr and BUN ，the protein expression levels of Bax ，Caspase-3 and Caspase- 9 in renal tissue were decreased significantly （P＜0.05 or P＜0.01）；there was the same chage trend and most with significant difference in above indexes of TFA low-dose group （P＜0.05 or P＜0.01）；the pathalogic changes of testis ，epididymis and renal tissue were improved significantly. CONCLUSIONS：TFA can significantly improve adenine-induced reproductive dysfunction model male rats. The mechanism may be associated with reducing hormone FSH and LH level ，the expression of apoptosis-related proteins in renal tissue ，while increasing the level of hormone T and E 2 and improving oxidant stress level. |
| 期刊： | 2020年第31卷第15期 |
| 作者： | 刘雯，侯晨辉，李江 |
| AUTHORS： | LIU Wen，HOU Chenhui ，LI Jiang |
| 关键字： | 沙苑子总黄酮；生殖功能障碍；性激素；氧化应激；机制；大鼠 |
| KEYWORDS： | Astragalus complanatus total flavonoids ；Reproductive dysfunction ；Horone；Oxidant stress ；Mechanism；Rat |
| 阅读数： | 280 次 |
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