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紫苏叶多糖对糖尿病模型小鼠胰腺组织氧化应激及PI3K/AKT/GLUT4信号通路的影响
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| 篇名： | 紫苏叶多糖对糖尿病模型小鼠胰腺组织氧化应激及PI3K/AKT/GLUT4信号通路的影响 |
| TITLE： | Effects of Purple frutescens Leaves Polysaccharides on Oxidative Stress and PI 3K/AKT/GLUT4 Signaling Pathway of Pancreatic Tissues in Diabetes Mellitus Model Mice |
| 摘要： | 目的：研究紫苏叶多糖对糖尿病（DM）模型小鼠胰腺组织氧化应激及磷酸肌醇-3-激酶（PI3K）/苏氨酸蛋白激酶（AKT）/葡萄糖转运蛋白4（GLUT4）信号通路的影响。方法：取60只小鼠，采用腹腔注射链脲佐菌素（60mg/kg）的方法复制糖尿病模型。将造模成功的40只小鼠随机分为模型组、二甲双胍组（阳性对照，200mg/kg）和紫苏叶多糖高、低剂量组（400、200mg/kg），每组10只；另取10只健康小鼠作为正常组（生理盐水）。每天灌胃给药1次，连续给药28d。实验期间，观察小鼠一般状况和体质量变化；进行口服葡萄糖耐量（OGTT）实验[测定灌胃40％葡萄糖溶液0、30、60、120min后的空腹血糖（FBG）水平]。末次给药后，测定小鼠血糖相关指标[FBG、空腹胰岛素（FINS）和胰岛素敏感指数（ISI）、胰岛素抵抗指数（IRI）]、血脂相关指标[血清中高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、总胆固醇（TC）、三酰甘油（TG）]和氧化应激相关指标[胰腺组织中丙二醛（MDA）含量和超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性]的变化，并测定小鼠胰腺组织中PI3K、磷酸化AKT（p-AKT）和GLUT4蛋白表达水平。结果：实验期间，与正常组比较，模型组小鼠行动迟缓，饲料消耗量和饮水量增多，体质量显著增加（P＜0.05）；给予葡萄糖0、30、60、120min后，小鼠的FBG水平均显著升高（P＜0.05）；末次给药后，血清中FINS、HDL-C含量和ISI以及胰腺组织中SOD、CAT、GSH-Px活性和PI3K、p-AKT、GLUT4蛋白表达水平均显著降低（P＜0.05），而血清中FBG、LDL-C、TC、TG含量和IRI以及胰腺组织中MDA含量均显著升高（P＜0.05）。与模型组比较，各给药组小鼠一般情况和OGTT情况均有好转；血清中FINS、HDL-C含量和ISI以及胰腺组织中SOD、CAT、GSH-Px活性和PI3K、p-AKT、GLUT4蛋白表达水平均显著升高（P＜0.05），而血清中FBG、LDL-C、TC、TG含量和IRI以及胰腺组织中MDA含量均显著降低（P＜0.05）。结论：紫苏叶多糖具有抗糖尿病作用，该作用可能与降低氧化应激水平和促进PI3K/AKT/GLUT4信号通路的活化有关。 |
| ABSTRACT： | OBJECTIVE：To study the effects of Purple frute scens leaves polysaccharides （PPLPs）on oxidative stress and PI3K/AKT/GLUT4 signaling pathway of pancreatic tissues in diabetes mellitus （DM）model mice. METHODS ：Totally 60 mice were given intraperitoneal injection of STZ （60 mg/kg）to induce DM model. The 40 successful modeling mice were randomly divided into model group ，metformin group （positive control ，200 mg/kg），PPLPs high-dose and low-dose groups （400，200 mg/kg），with 10 mice in each group. Another 10 healthy mice were selected as the normal group （normal saline ）. They were given relevant medicine intragastrically ，once a day ，for consecutive 28 days. During the experiment ，general information and body weight of mice were observed ；oral glucose tolerance （OGTT）test（determining FBG at 0，30，60，120 min after giving 40％ glucose solution ）was conducted. After last medication ，the changes of related blood glucose indexes （FBG，FINS，ISI，IRI）， blood lipid indexes （HDL-C，LDL-C，TC，TG）and oxidant stress indexes （MDA content and the activities of SOD ，CAT， GSH-Px）as well as the protein expressions of PI 3K，p-AKT and GLUT 4 in pancreatic tissue were determined. RESULTS ：During the experiment ，compared with normal group ，the mice were slow in action ，the feed consumption and water consumption increased，and body weight significantly increased in model group （P＜0.05）. 0，30，60，120 min after giving glucose ，the FBG content of mice were all increased significantly （P＜0.05）. After last medication ，the contents of FINS and HDL-C in serum as well as ISI ，the activities of SOD ，CAT and GSH-Px as well as the protein expressions of PI 3K，p-AKT and GLUT 4 in pancreatic tissue were all decreased significantly （P＜0.05）；the contents of FBG and LDL-C ，TC and TG in serum as well as IRI ， 疗。E-mail：sunguangping83@163.com MDA content in pancreatic tissue were all increased significantly（P＜0.05）. Compared with model group ，the general condition and OGTT of mice in each administration group was improved；the contents of FINS and HDL-C in serum as well as ISI ，the activities of SOD ，CAT and GSH-Px as well as the protein expressions of PI 3K，p-AKT and GLUT 4 in pancreatic tissue were all increased significantly （P＜0.05）；the contents of FBG，LDL-C，TC and TG in serum as well as IRI ，MDA content of pancreatic tissue were decreased significantly （P＜0.05）. CONCLUSIONS：PPLPs has anti-diabetic effects ，which are related to reducting oxidative stress level and promoting the activation of PI 3K/AKT/GLUT4 signaling pathway. |
| 期刊： | 2020年第31卷第15期 |
| 作者： | 孙广平，袁丽，方晓琳，杨海波 |
| AUTHORS： | SUN Guangping，YUAN Li，FANG Xiaolin，YANG Haibo |
| 关键字： | 紫苏叶多糖；氧化应激；PI3K/AKT/GLUT4信号通路；糖尿病；小鼠 |
| KEYWORDS： | Purple frutescens leaves polysaccharides ；Oxidative stress ；PI3K/AKT/GLUT4 signaling pathway ；Diabetes；Mice |
| 阅读数： | 405 次 |
| 本月下载数： | 9 次 |
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