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猪牙皂的萃取方法研究及萃取成分的GC-MS分析
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| 篇名： | 猪牙皂的萃取方法研究及萃取成分的GC-MS分析 |
| TITLE： | Study on the Extraction Technology of Fructus Gleditisae Abnormalis and GC-MS Analysis of Extracted Com- ponents |
| 摘要： | 目的：建立猪牙皂的萃取方法并优化，同时分析鉴定萃取物的化学成分。方法：采用CO2超临界流体萃取（SFE）法对猪牙皂进行萃取；在单因素试验的基础上，以萃取率为评价指标，萃取温度、萃取压强、萃取时间为考察因素，采用正交试验优化SFE工艺并进行验证。采用气质联用技术对萃取物的化学成分进行鉴定，并采用面积归一化法计算各成分的相对百分含量。结果：猪牙皂的最优SFE萃取工艺为萃取温度60℃、萃取压强300MPa、萃取时间15min；3次验证试验的平均萃取率为1.73％（RSD＝1.78％，n＝3）。共鉴定出猪牙皂萃取物中的48个成分，占萃取物总量的98.31％；其中，有机酸类成分最多，占总成分的36.99％；其次是生物碱类成分，占总成分的12.59％；主要成分包括棕榈酸（16.62％）、油酸（14.12％）、N-氨基四氢吡咯（9.79％）、2，6-二甲基辛烷-1，7-二烯-3-醇（5.95％）、四氢吡喃（3.83％）、香兰素（3.39％）等。结论：成功建立猪牙皂萃取的SFE法，萃取物主要为有机酸类成分。 |
| ABSTRACT： | OBJECTIVE：To establish and optimize a extraction method of Fructus Gleditsiae Abnormallis ，and to analyze and identify chemical components of the extract simultaneously. METHODS ：Fructus Gleditsiae Abnormallis was extracted with CO 2 supercritical fluid extraction （SFE）method. Based on single factor tests ，using extraction yield as index ，extraction temperature ， extraction pressure and extraction time as investigation factors ，SFE technology was optimized with orthogonal test ，and validation test was performed. Chemical components in the extract were identified by GC-MS. Relative percentage of each component was calculated with area normalization method. RESULTS ：The optimal SFE extraction technology of Fructus Gleditsiae Abnormallis was extraction temperature of 60 ℃，extraction pressure of 300 MPa and pression time of 15 min. Average extraction of 3 times of validation tests was 1.73％（RSD＝1.78％，n＝3）. The 48 components in the extracts of Fructus Gleditsiae Abnormallis were identified，which accounted for 98.31％ of the total amount of the extracts. The extracts of Fructus Gleditisae Abnormalis mainly included organic acids ，accounting for 36.99％，followed by alkaloids ，accounting for 12.59％ in total. Main components were palmitic acid （16.62％），oleic acid （14.12％），N-aminotetrahydropyrrole（9.79％），2，6-dimethyloctane-1，7-dien-3-ol（5.95％）， tetrahydropyran（3.83％），vanillin（3.39％），etc. CONCLUSIONS ：SFE method of Fructus Gleditisae Abnormalis is established successfully，and the extract is mainly organic acids. |
| 期刊： | 2020年第31卷第20期 |
| 作者： | 邓铋莉，董娜娜，王旭兰，罗成月，陈晓兰 |
| AUTHORS： | DENG Bili，DONG Nana，WANG Xulan，LUO Chengyue ，CHEN Xiaolan |
| 关键字： | 猪牙皂；CO2超临界流体萃取法；单因素试验；正交试验；气质联用技术 |
| KEYWORDS： | Fructus Gleditisae Abnormalis ；CO2 supercritical fluid extraction ；Single factor test ；Orthogonal test ；GC-MS |
| 阅读数： | 302 次 |
| 本月下载数： | 4 次 |
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