甘草饮片中抗氧化活性成分的快速筛选及鉴定
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篇名: 甘草饮片中抗氧化活性成分的快速筛选及鉴定
TITLE: Rapid Screening and Identification of Antioxidant Active Components in Glycyrrhiza uralensis Decoction Pieces
摘要: 目的:建立在线检测甘草饮片中抗氧化活性成分的方法,并对其进行鉴定。方法:通过检测1,1-二苯基-2-三硝基苯肼(DPPH)清除率来评价甘草饮片的抗氧化活性;采用在线高效液相色谱-紫外-1,1-二苯基-2-三硝基苯肼法(HPLC-UV-DPPH)筛选甘草饮片中的抗氧化活性成分;采用高效液相色谱-飞行时间高分辨质谱法(HPLC-TOF/MS)获取质谱数据并采用QualitiveAna-lystB06.00Build6.0.633.0软件进行分析,通过对比分析甘草的紫外吸收光谱、在线图谱、质谱信息及各化合物的保留时间、精确分子量,结合参考相关文献初步鉴定抗氧化活性成分,并进行验证。结果:8批甘草饮片的DPPH清除率为55.71%~60.17%,均可筛选出7种抗氧化活性成分,经鉴定分别为阿佛洛莫生、8-异戊烯基柚皮素、黄羽扇豆魏特酮、半甘草异黄酮B、3′,4′-二甲氧基-3-羟基-6-甲基黄酮、甘草宁E和甘草宁H。经验证,在线反应产生的倒峰峰面积与饮片的DPPH清除率呈正相关。结论:所建方法简单、准确,可用于快速筛选并鉴定甘草饮片的抗氧化活性成分,倒峰峰面积可用于评价甘草饮片的抗氧化活性成分大小。
ABSTRACT: OBJECTIVE:To establish a method for online detection of antioxidant active components in Glycyrrhiza uroalensis decoction pieces ,and to identify it. METHODS :The free radical scavenging rate of 1,1-diphenyl-2-trinitrobenzene hydrazine (DPPH)was determined to evaluate the antioxidant activity of G. uralensis decoction pieces. HPLC-UV-DPPH method was used to screen the anti oxidant active components of G. uralensis decoction pieces. HPLC-TOF/MS was used to obtain mass spectrum data and Qualitive Analyst B 06.00 Build 6.0.633.0 software was used to analyze data. Through contrast analysis of UV absorption spectrum,online chromatogram ,mass spectrum information of G. uralensis and the retention time of each compound ,accurate molecular weight ,antioxidant active components were identified by referring to relevant literature. Validation test was also conducted. RESULTS :DPPH free radical scavenging rate in 8 batches of G. uralensis decoction pieces ranged 55.71%-60.17%. Seven antioxidative active compounds ,including avolomotor ,8-isopentenyl naringin ,yellow lupulin weitone ,isoflavone B ,3′, 4′-dimethoxy3-hydroxy-6-methyl flavone ,glycyrrhizin E and glycyrrhizin H ,could be screened from G. uralensis decoction pieces. After validation ,the peak area of inverted peak generated by online reaction was positively correlated with DPPH free radical scavenging rate. CONCLUSIONS :Established method is simple and accurate ,and can be used to quickly screen and identify the main antioxidant components of G. uralensis decoction pieces ;the peak area of inverted peak can be used to evaluate the antioxidant active components of G. uralensis decoction pieces.
期刊: 2021年第32卷第02期
作者: 化敏,周倩,蒋海强,戴衍朋,石典花,王平,张乐林,周建永
AUTHORS: HUA Min, ZHOU Qian,JIANG Haiqiang,DAI Yanpeng,SHI Dianhua,WANG Ping,ZHANG Lelin,ZHOU Jianyong
关键字: 甘草;抗氧化活性成分;在线高效液相色谱-紫外-1,1-二苯基-2-三硝基苯肼法;高效液相色谱-飞行时间高分辨质谱法;1,
KEYWORDS: Glycyrrhiza uralensis; Antioxidant active component ; Online HPLC-UV-DPPH ; HPLC-TOF/MS; 1,
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