木香烃内酯对乳腺癌SK-BR-3细胞周期分布及凋亡的影响
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篇名: | 木香烃内酯对乳腺癌SK-BR-3细胞周期分布及凋亡的影响 |
TITLE: | Effects of Costunolide on Cell Cycle Distribution and Apoptosis of Breast Cancer SK-BR- 3 Cells |
摘要: | 目的:研究木香烃内酯(COS)对人乳腺癌SK-BR-3细胞周期分布及凋亡的影响,并探讨其机制。方法:将人乳腺癌SK-BR-3细胞分为空白对照组和COS10、20、30、40、50μmol/L组,用MTT法检测COS对细胞增殖的影响。另将细胞分为空白对照组和COS低、中、高浓度组(10、20、30μmol/L),培养24h后,采用流式细胞仪检测细胞周期分布情况,采用Hoechst33258荧光染色检测细胞凋亡情况,采用Westernblot法检测抑癌因子p53、胱天蛋白酶3(caspase-3)、B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)、细胞周期依赖性蛋白激酶抑制因子1A(p21)、周期蛋白依赖性激酶2(CDK2)和细胞周期蛋白E(cyclinE)蛋白的表达情况。结果:与空白对照组比较,COS可显著抑制SK-BR-3细胞的增殖(P<0.05或P<0.01),且呈浓度和时间依赖趋势。低、中、高浓度的COS均可诱导细胞凋亡并阻滞细胞周期于G1/S期(P<0.05或P<0.01);并可显著上调p53、caspase-3、Bax、p21蛋白的表达(P<0.05或P<0.01),显著下调Bcl-2、CDK2、cyclinE蛋白的表达(P<0.01)。结论:COS能抑制人乳腺癌SK-BR-3细胞的增殖,并诱导细胞凋亡和周期阻滞;其作用机制可能与调控p53/Bax/Bcl-2/caspase-3凋亡信号通路有关。 |
ABSTRACT: | OBJECTIVE:To study the effects of costunolide (COS)on the cell cycle distribution and apoptosis of human breast cancer SK-BR- 3 cells and its mechanism. METHODS :Human breast cancer SK-BR- 3 cells were divided into blank control group,and COS groups of 10,20,30,40,50 μmol/L. MTT assay was used to detect the effects of COS on cell proliferation. SK-BR-3 cells were divided into blank control group ,COS low ,medium and high concentration groups (10,20,30 μmol/L). After cultured for 24 h,flow cytometry was used to detect the distribution of cell cycle. Hoechst 33258 fluorescence staining was used to detect cell apoptosis. Western blot assay was used to detect the expression of p 53,caspase-3,Bcl-2,Bax,p21,CDK2 and cyclinE. RESULTS :Compared with blank control group ,COS could significantly inhibit the proliferation of SK-BR- 3 cells(P< 0.05 or P<0.01),and in a dose and time-dependent manner. Low ,medium and high concentrations of COS could induce cell apoptosis and arrest cell at G 1/S phase (P<0.05 or P<0.01),could significantly up-regulate the protein expression of p 53, caspase-3,Bax and p 21(P<0.05 or P<0.01),and could significantly down-regulate the protein expression of Bcl- 2,CDK2 and cyclinE(P<0.01). CONCLUSIONS :COS can inhibit the proliferation of human breast cancer SK-BR- 3 cells and induce cell apoptosis and cell cycle arrest. The mechanism may be related to the regulation of p 53/Bax/Bcl-2/caspase-3 apoptosis signal pathway. |
期刊: | 2021年第32卷第17期 |
作者: | 马强,陈洁,苗加伟,熊书,庞毅,邓雪松 |
AUTHORS: | MA Qiang,CHEN Jie,MIAO Jiawei,XIONG Shu,PANG Yi,DENG Xuesong |
关键字: | 木香烃内酯;SK-BR-3细胞;细胞增殖;细胞周期;细胞凋亡 |
KEYWORDS: | Costunolide;SK-BR-3 cells;Cell proliferation ;Cell cycle ;Cell apoptosis |
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