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壮药华佗豆不同极性部位HPLC指纹图谱的建立及其镇痛抗炎作用的谱效关系
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| 篇名： | 壮药华佗豆不同极性部位HPLC指纹图谱的建立及其镇痛抗炎作用的谱效关系 |
| TITLE： | Establishment of HPLC Fingerprint of Different Polar Parts of Zhuang Medicine Calonyction muricatum and Spectrum-effect Relationship of Its Analgesic and Anti-inflammatory Effects |
| 摘要： | 目的：建立壮药华佗豆不同极性部位的高效液相色谱（HPLC）指纹图谱，并研究其与镇痛抗炎作用的谱效关系。方法：制备华佗豆总部位及乙酸乙酯、正丁醇、水部位。采用HPLC法进行测定并结合《中药色谱指纹图谱相似度评价系统》（2012A版）建立不同极性部位的HPLC指纹图谱，并进行共有峰指认。以小鼠扭体次数和耳肿胀度为镇痛抗炎指标，考察华佗豆不同极性部位的镇痛抗炎活性；采用灰色关联度分析法、双关变量分析法、偏最小二乘法（PLS）分析各极性部位HPLC指纹图谱中共有峰与镇痛抗炎指标的相关性。结果：华佗豆不同极性部位共有11个共有峰，指认了5个成分，分别为新绿原酸（3号峰）、绿原酸（5号峰）、隐绿原酸（6号峰）、异绿原酸A（10号峰）、异绿原酸C（11号峰）。经灰色关联度分析发现，所有共有峰与镇痛抗炎作用的关联度均大于0.6（除6号峰与镇痛作用的关联度外），呈相关性；3、7、10号峰与镇痛抗炎作用的关联度均大于0.8，呈高度相关性。经双关变量分析发现，1、3、4、7、9、10、11号峰与镇痛抗炎作用的相关系数均大于0.6，呈相关性。经PLS分析发现，1、3、4、7、9、10、11号峰对镇痛抗炎作用的影响较大。结论：建立了华佗豆不同极性部位的HPLC指纹图谱并指认了5个共有峰成分；新绿原酸、异绿原酸A、异绿原酸C以及1、4、7、9号峰所代表的化学成分可能是华佗豆发挥镇痛抗炎作用的药效物质。 |
| ABSTRACT： | OBJECTIVE：To establish HPLC fingerprints of different polar parts of Zhuang medicine Calonyction muricatum ， and to study its spectrum-effect relationship with analgesic and anti-inflammatory effects. METHODS ：The total part ，ethyl acetate part，n-butanol part and water part of C. muricatum were prepared. HPLC fingerprints of different polar parts were established by HPLC method combined with the Similarity Evaluation System of TCM Chromatogramtic Fingerprint （2012A），and the common peaks were identified. Using writhing times and ear swelling degree in mice as analgesic and anti-inflammatory indexes ，analgesic and anti-inflammatory activity of different polar parts of C. muricatum were investigated. The correlation of the common peaks of HPLC fingerprint with analgesic and anti-inflammatory indexes was analyzed by grey correlation analysis ，bivariate correlation analysis and partial least square （PLS） method. RESULTS ： There were 11 common peaks for the different polar parts of C. muricatum ，and 5 components were identified by reference comparison，i.e. neochlorogenic acid （peak 3），chlorogenic acid （peak 5）， cryptochlorogenic acid （peak 6）， isochlorogenic acid A （peak 10），isochlorogenic acid C （peak 11）. The grey correlation analysis showed that the correlation between all common peaks and analgesic and anti- inflammatory effects were greater than 0.6 （except the correlation between peak 6 and analgesic effects ），showing correlation relationship ；the correlation of peaks 3，7 and 10 with analgesic and anti-inflammatory effects were all greater than 0.8，which was highly related. Bivariate correlation analysis showed that the correlation of peak 1，3，4，7，9，10，11 with analgesic and anti-inflammatory effects were all greater than 0.6，showing correlation relationship. PLS method showed that peaks 1，3，4，7，9，10，11 contributed greatly to playing an analgesic and anti-inflammatory role. CONCLUSIONS ：HPLC fingerprints of different polar parts of C. muricatum is established and five common peak components were identified. Neochlorogenic acid ，isochlorogenic acid A ，isochlorogenic acid C and chemical components represented by peaks 1，4，7，9 may be the pharmacodynamic substances of C. muricatum to exert analgesic and anti-inflammatory effects. |
| 期刊： | 2021年第32卷第17期 |
| 作者： | 林婧，梁洁，黄春燕，韦金玉，黄冬芳，胡珏，祁金丽，陈辉华 |
| AUTHORS： | LIN Jing，LIANG Jie，HUANG Chunyan ，WEI Jinyu，HUANG Dongfang ，HU Jue，QI Jinli，CHEN Huihua |
| 关键字： | 华佗豆；镇痛抗炎；指纹图谱；谱效关系 |
| KEYWORDS： | Calonyction muricatum ；Analgesic and anti-inflammatory ；Fingerprint；Spectrum-effect relationship |
| 阅读数： | 229 次 |
| 本月下载数： | 4 次 |
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